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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1993-5-27
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pubmed:abstractText |
Two kinds of unrelated African swine fever virus proteins of 220 kDa have been identified by means of two-dimensional gel electrophoresis and immunoprecipitation analysis. One species, named pp220 and identified as the precursor of the major structural protein p150, was found to be a moderately acidic protein (pl near 7) expressed after the replication of the viral DNA. The second species, a cluster of 220-kDa proteins with slightly different isoelectric points (pl ranging from 5 to 6), was found to be a homooligomeric complex formed by an early 32-kDa protein. This component was identified as the viral phosphoprotein p32, the most immunogenic early protein of African swine fever virus. A detailed characterization of its oligomeric structure is reported.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0042-6822
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
194
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
284-93
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8480423-African Swine Fever Virus,
pubmed-meshheading:8480423-Animals,
pubmed-meshheading:8480423-Cross-Linking Reagents,
pubmed-meshheading:8480423-Disulfides,
pubmed-meshheading:8480423-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:8480423-Macrophages,
pubmed-meshheading:8480423-Phosphoproteins,
pubmed-meshheading:8480423-Protein Conformation,
pubmed-meshheading:8480423-Protein Precursors,
pubmed-meshheading:8480423-Swine,
pubmed-meshheading:8480423-Vero Cells,
pubmed-meshheading:8480423-Viral Proteins,
pubmed-meshheading:8480423-Viral Structural Proteins
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pubmed:year |
1993
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pubmed:articleTitle |
Characterization of two African swine fever virus 220-kDa proteins: a precursor of the major structural protein p150 and an oligomer of phosphoprotein p32.
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pubmed:affiliation |
Centro de Biología Molecular (C.S.I.C.-U.A.M.), Facultad de Ciencias, Universidad Autónoma, Cantoblancó, Madrid, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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