Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3 Pt 1
|
| pubmed:dateCreated |
1993-4-29
|
| pubmed:abstractText |
We assessed the effects of cultured human promyelocytic leukemia (HL-60) cells and polymorphonuclear leukocytes (neutrophils) isolated from peripheral human blood on tracheal smooth muscle responsiveness in 40 male Hartley guinea pigs. Undifferentiated HL-60 cells (16-25 passages) were activated in vitro by incubation with 1 microM f-Met-Leu-Phe (fMLP), and force of contraction was measured isometrically using an in situ preparation of tracheal smooth muscle. Increasing concentrations of acetylcholine (ACh; 10(-10) to 10(-6) mol/cm2 tracheal surface) were applied topically to the epithelial surface pretreated with 4 x 10(6) fMLP-activated HL-60 cells, 4 x 10(6) fMLP-activated neutrophils, 4 x 10(6) sham-activated HL-60 cells, fMLP+vehicle, or vehicle control. Topical application of fMLP-activated HL-60 cells caused a maximum active tension (AT) of 1.13 +/- 0.2 g/cm after 5 min; fMLP-activated neutrophils, sham-activated HL-60 cells, or fMLP+vehicle had no effect. The fMLP-activated HL-60 cells also caused substantial augmentation of tracheal contraction to ACh (P < 0.05 vs. sham-activated cells for all concentrations > 10(-9) mol/cm2). Although fMLP treatment caused 247 +/- 28% increase from baseline level in O2-. production, neither direct contraction nor augmentation of muscarinic stimulation was demonstrated after topical application of 4 x 10(6) neutrophils. In 12 other preparations, fMLP-activated HL-60 cells were pretreated with either 10 microM indomethacin (Indo) or 100 microM A63162, a 5-lipoxygenase inhibitor. Pretreatment with Indo caused complete blockade of direct tracheal contraction and 88 +/- 13% blockade of muscarinic augmentation; there was no effect after A63162.(ABSTRACT TRUNCATED AT 250 WORDS)
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetamides,
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/Indomethacin,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoxygenase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/N-Formylmethionine...,
http://linkedlifedata.com/resource/pubmed/chemical/N-hydroxy-N-(1-(4-(phenylmethoxy)phe...,
http://linkedlifedata.com/resource/pubmed/chemical/Phenyl Ethers,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin-Endoperoxide Synthases
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0002-9513
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
264
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
L222-8
|
| pubmed:dateRevised |
2010-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:8460711-Acetamides,
pubmed-meshheading:8460711-Acetylcholine,
pubmed-meshheading:8460711-Animals,
pubmed-meshheading:8460711-Bronchoconstriction,
pubmed-meshheading:8460711-Cell Communication,
pubmed-meshheading:8460711-Cell Survival,
pubmed-meshheading:8460711-Guinea Pigs,
pubmed-meshheading:8460711-Humans,
pubmed-meshheading:8460711-Immunohistochemistry,
pubmed-meshheading:8460711-Indomethacin,
pubmed-meshheading:8460711-Leukemia, Myeloid,
pubmed-meshheading:8460711-Lipoxygenase,
pubmed-meshheading:8460711-Lipoxygenase Inhibitors,
pubmed-meshheading:8460711-Male,
pubmed-meshheading:8460711-Muscle, Smooth,
pubmed-meshheading:8460711-N-Formylmethionine Leucyl-Phenylalanine,
pubmed-meshheading:8460711-Neutrophils,
pubmed-meshheading:8460711-Phenyl Ethers,
pubmed-meshheading:8460711-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:8460711-Trachea,
pubmed-meshheading:8460711-Tumor Cells, Cultured
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Activation of tracheal smooth muscle responsiveness by fMLP-treated HL-60 cells and neutrophils.
|
| pubmed:affiliation |
Department of Medicine, University of Chicago, Illinois 60637.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|