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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1993-4-9
|
| pubmed:abstractText |
A human monoclonal antibody, YJ-37 (IgM) was generated through the fusion of human B lymphoblastoid cell line HO-323 with the regional lymph node lymphocytes from a colonic cancer patient who was treated with a local immunotherapy. This antibody was purified and conjugated with biotin, after which direct immunohistochemical staining was performed. The results revealed that YJ-37 selectively reacted with colonic cancer (7/19), gastric cancer (3/6), endometrial cancer (1/2) and colonic adenoma (7/13), but not with normal epithelia. Membrane immunofluorescence and FACS analysis also showed that YJ-37 bound to tumor cell surfaces. Furthermore, the chemical structure of the antigen defined by YJ-37 was analyzed by means of thin-layer chromatography immunostaining and ELISA. The results indicated that YJ-37 reacted with sialylated lacto-series carbohydrate chains, which have been reported to accumulate in cancer cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0910-5050
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
84
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
N
|
| pubmed:pagination |
75-82
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8449830-Adenoma,
pubmed-meshheading:8449830-Antibodies, Monoclonal,
pubmed-meshheading:8449830-B-Lymphocytes,
pubmed-meshheading:8449830-Carbohydrate Conformation,
pubmed-meshheading:8449830-Carbohydrate Sequence,
pubmed-meshheading:8449830-Cell Fusion,
pubmed-meshheading:8449830-Cell Line,
pubmed-meshheading:8449830-Colonic Neoplasms,
pubmed-meshheading:8449830-Endometrial Neoplasms,
pubmed-meshheading:8449830-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:8449830-Female,
pubmed-meshheading:8449830-Fibrinogen,
pubmed-meshheading:8449830-Glycolipids,
pubmed-meshheading:8449830-Humans,
pubmed-meshheading:8449830-Immunotherapy,
pubmed-meshheading:8449830-Lymph Nodes,
pubmed-meshheading:8449830-Lymphocytes,
pubmed-meshheading:8449830-Molecular Sequence Data,
pubmed-meshheading:8449830-Picibanil,
pubmed-meshheading:8449830-Stomach Neoplasms
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
A cancer-reactive human monoclonal antibody derived from a colonic cancer patient treated with local immunotherapy.
|
| pubmed:affiliation |
Second Department of Surgery, Osaka University Medical School.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|