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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1993-2-24
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pubmed:databankReference |
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pubmed:abstractText |
We have molecularly cloned and sequenced a cDNA for a rat liver nucleoporin with a molecular mass of 152.8 kd, termed nup153, that shares a repetitive degenerate pentapeptide motif with a subgroup of nucleoporins of yeast and vertebrates. However, its most striking feature is a novel 4-fold repeat of a Cys2-Cys2-type zinc finger motif. When expressed in E. coli, the zinc finger domain of nup153 binds DNA in a zinc-dependent fashion. Immunoelectron microscopy localized nup153 exclusively to the nucleoplasmic side of the nuclear pore complex. We suggest that nup153 recognizes a specific DNA sequence to organize the genome three-dimensionally and to gate transcribable genes to nuclear pore complexes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
72
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
29-38
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8422679-Amino Acid Sequence,
pubmed-meshheading:8422679-Animals,
pubmed-meshheading:8422679-Antibodies, Monoclonal,
pubmed-meshheading:8422679-Base Sequence,
pubmed-meshheading:8422679-Cell Line,
pubmed-meshheading:8422679-Cloning, Molecular,
pubmed-meshheading:8422679-DNA,
pubmed-meshheading:8422679-Fluorescent Antibody Technique,
pubmed-meshheading:8422679-Immunoblotting,
pubmed-meshheading:8422679-Membrane Glycoproteins,
pubmed-meshheading:8422679-Microscopy, Immunoelectron,
pubmed-meshheading:8422679-Molecular Sequence Data,
pubmed-meshheading:8422679-Nuclear Pore Complex Proteins,
pubmed-meshheading:8422679-Nuclear Proteins,
pubmed-meshheading:8422679-Protein Binding,
pubmed-meshheading:8422679-Rats,
pubmed-meshheading:8422679-Sequence Homology, Amino Acid,
pubmed-meshheading:8422679-Zinc Fingers
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pubmed:year |
1993
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pubmed:articleTitle |
A nuclear pore complex protein that contains zinc finger motifs, binds DNA, and faces the nucleoplasm.
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pubmed:affiliation |
Howard Hughes Medical Institute, Rockefeller University, Laboratory of Cell Biology, New York, New York 10021.
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pubmed:publicationType |
Journal Article
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