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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1993-11-1
|
| pubmed:abstractText |
The MSI3 gene was isolated as a multicopy suppressor of the heat shock-sensitive phenotype of the ira1 mutation, which causes hyperactivation of the RAS-cAMP pathway. Overexpression of MSI3 also suppresses the heat shock-sensitive phenotype of the bcy1 mutant. Determination of the DNA sequence of MSI3 revealed that MSI3 can encode a 77.4 kDa protein related to the HSP70 family. The amino acid sequence of Msi3p is about 30% identical to that of the Ssa1p of Saccharomyces cerevisiae. This contrasts with the finding that members of the HSP70 family generally show at least 50% amino acid identity. The consensus nucleotide sequence of the heat shock element (HSE) was found in the upstream region of MSI3. Moreover, the steady-state levels of the MSI3 mRNA and protein were increased upon heat shock. These results indicate that the MSI3 gene encodes a novel HSP70-like heat shock protein. Disruption of the MSI3 gene was associated with a temperature sensitive growth phenotype but unexpectedly, thermotolerance was enhanced in the disruptant.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Fungal,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Heat-Shock Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/ras Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0026-8925
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
240
|
| pubmed:geneSymbol |
MSI3
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
323-32
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8413180-Amino Acid Sequence,
pubmed-meshheading:8413180-Base Sequence,
pubmed-meshheading:8413180-Chromosome Mapping,
pubmed-meshheading:8413180-Chromosomes, Fungal,
pubmed-meshheading:8413180-Cloning, Molecular,
pubmed-meshheading:8413180-Cyclic AMP,
pubmed-meshheading:8413180-DNA, Fungal,
pubmed-meshheading:8413180-Fungal Proteins,
pubmed-meshheading:8413180-Genes, Fungal,
pubmed-meshheading:8413180-Genes, Suppressor,
pubmed-meshheading:8413180-Heat-Shock Proteins,
pubmed-meshheading:8413180-Molecular Sequence Data,
pubmed-meshheading:8413180-Mutation,
pubmed-meshheading:8413180-Phenotype,
pubmed-meshheading:8413180-Saccharomyces cerevisiae,
pubmed-meshheading:8413180-Sequence Homology, Amino Acid,
pubmed-meshheading:8413180-Signal Transduction,
pubmed-meshheading:8413180-Temperature,
pubmed-meshheading:8413180-ras Proteins
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
MSI3, a multicopy suppressor of mutants hyperactivated in the RAS-cAMP pathway, encodes a novel HSP70 protein of Saccharomyces cerevisiae.
|
| pubmed:affiliation |
Department of Biology, Faculty of Science, University of Tokyo, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|