Linked Life Data

8402387

Source:http://linkedlifedata.com/resource/pubmed/id/8402387

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
1993-11-4
pubmed:abstractText
The influence of repeated sampling by the biopsy technique on skeletal muscle's metabolic and force-output responses was studied using the in situ canine gastrocnemius preparation. The left muscle was stimulated (8 V, 0.2 ms) for 1 h at 3 Hz. In the biopsy series (n = 9) muscle samples were taken at rest, and at 0.5, 2, 5, 15, 30, 45, and 60 min of stimulation. In the control series (n = 8) the left and right muscles were quick-frozen in N2 immediately after the 60 min of stimulation. The two series were not different in blood flow, VO2, arterial or venous [H+], muscle glycogen, or lactate release throughout the 60 min of activity. The lactate release was transient and was associated with an accumulation of intramuscular lactate and a period of rapid glycogenolysis. The biopsy series had a modest but significantly (p < 0.05) higher muscle lactate concentration both at rest and at the end of the contractions. The biopsy series also had less (p < 0.05) tension development throughout the hour; however, the O2 cost per unit of tension development was not different between groups, nor was the rate of tension decline over time different. This together with the similarities in perfusion, carbohydrate use, and lactate metabolism suggests that repeated biopsies had minimal impact on the muscle. The technique allows the collection of data over time; this improves the detail of experiments and means that fewer animals are required for a study.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0008-4212
pubmed:author
pubmed:issnType
Print
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
241-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Active skeletal muscle metabolism and tension production: the influence of biopsies.
pubmed:affiliation
School of Human Biology, University of Guelph, ON, Canada.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't