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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1993-10-4
|
| pubmed:abstractText |
We demonstrate that DNA-binding protein extracts can be effectively prepared directly from tissue culture cells preserved under liquid nitrogen without returning the cells to culture. We prepared DNA-binding protein extracts directly upon thawing of T47D, Jurkat and CAC-L153S cell lines after storage in liquid nitrogen for periods of up to one year. Our results show that DNA binding of a repressor of mouse mammary tumor virus transcription in these extracts is indistinguishable from binding activity in similar extracts prepared from cells maintained in culture.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0736-6205
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
15
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
59-62, 64
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8395863-Base Sequence,
pubmed-meshheading:8395863-Binding Sites,
pubmed-meshheading:8395863-Cell Line,
pubmed-meshheading:8395863-Cryopreservation,
pubmed-meshheading:8395863-DNA, Viral,
pubmed-meshheading:8395863-DNA-Binding Proteins,
pubmed-meshheading:8395863-Mammary Tumor Virus, Mouse,
pubmed-meshheading:8395863-Molecular Sequence Data,
pubmed-meshheading:8395863-Nuclear Proteins,
pubmed-meshheading:8395863-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:8395863-Transcription, Genetic
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Extraction of DNA-binding proteins from cryogenically preserved cells.
|
| pubmed:affiliation |
Department of Medicine, University of Ottawa Moses and Rose Loeb Institute for Medical Research, Ottawa Civic Hospital, Ontario, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|