Linked Life Data

8381606

Source:http://linkedlifedata.com/resource/pubmed/id/8381606

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1 Pt 2
pubmed:dateCreated
1993-3-5
pubmed:abstractText
The relationship between Ca2+ and intracellular pH (pHi) was studied in the rabbit collecting duct from the inner stripe of the outer medulla (OMCDi). When bath Ca2+ was lowered [1 mM Ca2+ plus 2 mM ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA)] intracellular Ca2+ concentration ([Ca2+]i) decreased by 38.3 +/- 6.2% from 265 +/- 25 to 165 +/- 25 nm (n = 4). Lowering bath Ca2+ produced a biphasic response in pHi, transient acidification (from 6.93 +/- 0.06 to 6.80 +/- 0.06) followed by a return of pHi to levels slightly higher than control (pHi = 6.97 +/- 0.06). The acidification was not blocked by bath amiloride or 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) or lumen N-ethylmaleimide (NEM). However, bath amiloride significantly reduced the initial rate of pHi recovery after acidification (0.70 pH U/min in control vs. 0.39 pH U/min with amiloride). Lanthanum, a Ca2+ pump inhibitor, completely inhibited the acidification. Moreover, lowering bath temperature to 20 degrees C also inhibited the acidification. Acidification was blocked when the bath pH was raised to 8.0, whereas lowering bath Ca2+ markedly acidified cells when bath pH was lowered to 6.0 (delta pHi = 0.28 +/- 0.01). We also measured the transepithelial (Vt) and basolateral (Vbl) membrane voltages. Lowering bath Ca2+ depolarized Vt and Vbl by 12.7 +/- 4.9 and 6.0 +/- 2.0 mV, respectively. In summary, 1) lowering bath Ca2+ caused a biphasic response in pHi of OMCDi cells, and 2) the acidification phase is due to H+ influx associated with the Ca2+ pump.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
264
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
F88-93
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Evidence for basolateral membrane Ca2+/H+ exchange in outer medullary collecting duct.
pubmed:affiliation
Department of Medicine, Vanderbilt University, Nashville, Tennessee.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.