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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
1993-10-1
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pubmed:abstractText |
With increasing knowledge about the causal role of genetic defects in clinical diseases the necessity is apparent to have procedures for rapid diagnosis of point mutations. We developed a PCR-based technique, whereby both normal and mutant alleles can be amplified in the same reaction tube, using different length allele-specific primers. Furthermore the allele-specific primers introduce additional deliberate differences into the allelic PCR-products that drastically reduce crossreactions in subsequent cycles. This mutagenesis separates the amplification reactions of the alleles performed in the same tube. Subsequent identification of the PCR-products is done by gel electrophoresis and shows at least one of the two allelic products. Therefore, in addition to simple handling, MS-PCR provides a within-assay quality control for the exclusion of false negative results. The feasibility of this technique has been tested using six different mutations. The high sensitivity of MS-PCR also allows screening for mutation carriers in pooled DNA samples.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-1349197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-1379414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-1579465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-1680583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-1986365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-2164382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-2179874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-2280177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-2308948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-2310429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-2479983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-2563166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-2578002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-2655589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-2717399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-2785681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-2987927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-3036793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-3477815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-3759943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-3773199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-3797244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8367277-8432868
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3623-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1993
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pubmed:articleTitle |
Mutagenically separated PCR (MS-PCR): a highly specific one step procedure for easy mutation detection.
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pubmed:affiliation |
Institut für Arterioskleroseforschung, Münster, Germany.
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pubmed:publicationType |
Journal Article
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