Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1993-10-1
|
| pubmed:abstractText |
Three rhamnose-binding lectins were purified from the roe of Osmerus eperlanus mordax (olive rainbow smelt) by affinity chromatography and ion-exchange chromatography. The apparent molecular weights of Osmerus eperlanus mordax lectin (OML) -1, -2 and -3 were 25000, 32000 and 26000, respectively, on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions. On native PAGE, these three lectins showed different migration patterns (Rm value; 0.37, 0.53 and 0.66, respectively). OMLs agglutinated rabbit and human type B erythrocytes and sarcoma 180 cells, but not human type A and O erythrocytes and AH109A cells. The most effective monosaccharide inhibitor was L-rhamnose. L-Mannose and D-galactose were also good inhibitors. Furthermore, OML-induced hemagglutination was inhibited more strongly by melibiose or raffinose rather than lactose or lactulose. Therefore, OMLs are L-rhamnose/alpha-D-galactosyl type lectins. OMLs did not require a detergent, when extracted from crude material, and Ca2+, Mg2+, EDTA and dithiothreitol were not necessary for the OML-induced hemagglutination activities. The OMLs had similar N-terminal amino acid sequences.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0918-6158
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
16
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
239-43
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8364467-Amino Acid Sequence,
pubmed-meshheading:8364467-Animals,
pubmed-meshheading:8364467-Carbohydrates,
pubmed-meshheading:8364467-Chromatography, Affinity,
pubmed-meshheading:8364467-Chromatography, Ion Exchange,
pubmed-meshheading:8364467-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8364467-Fishes,
pubmed-meshheading:8364467-Hemagglutination Inhibition Tests,
pubmed-meshheading:8364467-Hemagglutination Tests,
pubmed-meshheading:8364467-Hydrolysis,
pubmed-meshheading:8364467-Lectins,
pubmed-meshheading:8364467-Molecular Sequence Data,
pubmed-meshheading:8364467-Molecular Weight,
pubmed-meshheading:8364467-Ovum,
pubmed-meshheading:8364467-Rabbits,
pubmed-meshheading:8364467-Rhamnose,
pubmed-meshheading:8364467-Sarcoma 180,
pubmed-meshheading:8364467-Tumor Cells, Cultured
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Three rhamnose-binding lectins from Osmerus eperlanus mordax (olive rainbow smelt) roe.
|
| pubmed:affiliation |
Cancer Research Institute, Tohoku College of Pharmaceutical Sciences, Sendai, Japan.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|