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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5-6
|
| pubmed:dateCreated |
1993-8-5
|
| pubmed:abstractText |
Candida parapsilosis shows a wide intraspecies variation in chromosome/homolog size distribution. As a prerequisite for delineating modes of transmission, we have undertaken an analysis of genetic variation at different levels. In the present study we have observed that a majority of isolates display similar electrophoretic karyotype patterns consistent for the species, with variations in the smaller group of chromosomes. In two strains we observed phenotypic "switching"; one of these also exhibited a mixed karyotypic subpopulation. In contrast, a few isolates displayed a greater degree of chromosome/homolog size variation. We also observed, through randomly amplified polymorphic DNA (RAPD) analysis, results consistent with those of pulsed-field electrophoresis. Isolates displaying a high degree of chromosome/homolog variation also displayed a high degree of variation in genomic "fingerprints". Polymorphisms, although present, were much reduced in the majority of isolates. These parallel observations suggest a common underlying mechanism. Our results are consistent with the hypothesis that chromosome-sized variations in C. parapsilosis are due to random genetic events. A similar mechanism has been hypothesized for the taxonomically related yeast Candida albicans.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0172-8083
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
23
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
463-7
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:8319303-Base Sequence,
pubmed-meshheading:8319303-Candida,
pubmed-meshheading:8319303-Candidiasis,
pubmed-meshheading:8319303-DNA, Fungal,
pubmed-meshheading:8319303-Electrophoresis, Gel, Pulsed-Field,
pubmed-meshheading:8319303-Gene Amplification,
pubmed-meshheading:8319303-Genetic Variation,
pubmed-meshheading:8319303-Humans,
pubmed-meshheading:8319303-Karyotyping,
pubmed-meshheading:8319303-Molecular Sequence Data,
pubmed-meshheading:8319303-Phenotype
|
| pubmed:articleTitle |
Genomic heterogeneity in the yeast Candida parapsilosis.
|
| pubmed:affiliation |
Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Center for Disease Control, Atlanta, GA 30333.
|
| pubmed:publicationType |
Journal Article
|