Linked Life Data

8262567

Source:http://linkedlifedata.com/resource/pubmed/id/8262567

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6-7
pubmed:dateCreated
1994-1-27
pubmed:abstractText
Mononuclear cell elicitation has gained renewed interest with the discovery of a supergene family of small polypeptide chemotactic cytokines (< 10 kD). These chemotactic cytokines have been divided into the C-X-C and C-C chemokine families depending upon whether the first two conserved cysteine amino acid residues are separated by one amino acid or are in juxtaposition, respectively. A salient feature of the C-C chemokine family is their ability to induce both monocyte and lymphocyte chemotaxis. Although monocyte and lymphocyte migration in vitro is measured in chemotactic bioassays, this technique often fails to determine the specific quantitative contribution of a chemotaxin to a biological specimen. Our laboratory has developed two sensitive and specific sandwich ELISAs for the detection of macrophage inflammatory protein-1 alpha and beta (MIP-1 alpha and MIP-1 beta). The lower threshold for detection of both MIP-1 alpha and MIP-1 beta was 100 pg/ml, and both of these ELISAs were efficacious for the detection of MIP-1 alpha and MIP-1 beta in conditioned media from pulmonary fibroblasts, monocytes, neutrophils, and a pulmonary epithelial cell line. The development of these ELISAs will allow the measurement of MIP-1 alpha and MIP-1 beta from biologically relevant fluids and ascertain whether these two C-C chemokines are present in disease.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0882-0139
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
441-9
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:articleTitle
Specific ELISAs for the detection of human macrophage inflammatory protein-1 alpha and beta.
pubmed:affiliation
Department of Internal Medicine, University of Michigan Medical School, Ann Arbor 48109-0360.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't