| pubmed-article:8142465 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8142465 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:8142465 | lifeskim:mentions | umls-concept:C0032105 | lld:lifeskim |
| pubmed-article:8142465 | lifeskim:mentions | umls-concept:C0141947 | lld:lifeskim |
| pubmed-article:8142465 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:8142465 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:8142465 | pubmed:dateCreated | 1994-5-3 | lld:pubmed |
| pubmed-article:8142465 | pubmed:abstractText | Selenoprotein P was partially purified (> 1000-fold) from human plasma in four chromatographic steps using 75Se-labeled selenoprotein P secreted by HepG2 cells in culture as a marker. The purified preparation was injected into mice and monoclonal antibodies, which precipitated the labeled protein, were generated. Neither of two different monoclonal antibodies had cross-reactivity with plasma from five animal species. Antibodies were coupled to agarose, and selenoprotein P was purified from human plasma by immunoaffinity chromatography followed by chromatography on heparin agarose. With two different matrix-bound monoclonal antibodies, the purification procedure gave two bands on SDS-PAGE with mobilities corresponding to 61 and 55 kDa. Both bands stained for carbohydrate and showed increased electrophoretic mobility after enzymatic deglycosylation. Immunoaffinity chromatography removed approx. one-third of the selenium from plasma or 0.4 mumol Se/l at a total selenium concentration of 1.1 mumol/l, indicating that selenoprotein P constituted this proportion of total plasma selenium in healthy US blood donors. | lld:pubmed |
| pubmed-article:8142465 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8142465 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8142465 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8142465 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8142465 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8142465 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8142465 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8142465 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8142465 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8142465 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8142465 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8142465 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8142465 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:8142465 | pubmed:issn | 0006-3002 | lld:pubmed |
| pubmed-article:8142465 | pubmed:author | pubmed-author:AkessonBB | lld:pubmed |
| pubmed-article:8142465 | pubmed:author | pubmed-author:BurkR FRF | lld:pubmed |
| pubmed-article:8142465 | pubmed:author | pubmed-author:BelletSS | lld:pubmed |
| pubmed-article:8142465 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8142465 | pubmed:day | 16 | lld:pubmed |
| pubmed-article:8142465 | pubmed:volume | 1204 | lld:pubmed |
| pubmed-article:8142465 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8142465 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8142465 | pubmed:pagination | 243-9 | lld:pubmed |
| pubmed-article:8142465 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8142465 | pubmed:meshHeading | pubmed-meshheading:8142465-... | lld:pubmed |
| pubmed-article:8142465 | pubmed:meshHeading | pubmed-meshheading:8142465-... | lld:pubmed |
| pubmed-article:8142465 | pubmed:meshHeading | pubmed-meshheading:8142465-... | lld:pubmed |
| pubmed-article:8142465 | pubmed:meshHeading | pubmed-meshheading:8142465-... | lld:pubmed |
| pubmed-article:8142465 | pubmed:meshHeading | pubmed-meshheading:8142465-... | lld:pubmed |
| pubmed-article:8142465 | pubmed:meshHeading | pubmed-meshheading:8142465-... | lld:pubmed |
| pubmed-article:8142465 | pubmed:meshHeading | pubmed-meshheading:8142465-... | lld:pubmed |
| pubmed-article:8142465 | pubmed:meshHeading | pubmed-meshheading:8142465-... | lld:pubmed |
| pubmed-article:8142465 | pubmed:meshHeading | pubmed-meshheading:8142465-... | lld:pubmed |
| pubmed-article:8142465 | pubmed:meshHeading | pubmed-meshheading:8142465-... | lld:pubmed |
| pubmed-article:8142465 | pubmed:meshHeading | pubmed-meshheading:8142465-... | lld:pubmed |
| pubmed-article:8142465 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8142465 | pubmed:articleTitle | Purification of selenoprotein P from human plasma. | lld:pubmed |
| pubmed-article:8142465 | pubmed:affiliation | Department of Medicine, Vanderbilt University, Nashville, TN 37232-2279. | lld:pubmed |
| pubmed-article:8142465 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8142465 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8142465 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:6414 | entrezgene:pubmed | pubmed-article:8142465 | lld:entrezgene |
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