Linked Life Data

811261

Source:http://linkedlifedata.com/resource/pubmed/id/811261

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1976-2-13
pubmed:abstractText
The kinetic parameters of microsomal UDPglucuronyltransferase (EC 2.4.1.17) were compared after treatment of microsomes with phospholipase A and Triton X-100. Treatments with phospholipase A and Triton had differential effects on activities at V (assayed in both forward and reverse directions), the affinity of the enzyme for UDPglucuronic acid and UDP, the specificity of binding of UDPsugars, and the sensitivity of the enzyme to treatment with UDP-N-acetylgulcosamine. Comparisons of lysophosphatide, Triton, and cholate-activated forms indicated that different types of detergents activate by separate mechanisms. These data are taken as evidence that selective types of changes of the composition and structure of microsomal lipids lead to selective types of changes of the properties of UDPglucuronyltransferase. Activation of UDPglucuronyltransferase in response to different types of perturbations of the membrane lipids is thus a specific process. It was found that constraint on the maximal potential activity of the reverse reaction catalyzed by UDPglucuronyltransferase is more extensive in untreated microsomes than constraint on the forward reaction. Also, the "activated forms" of UDPglucuronyltransferase have less activity than the untreated enzyme if assays are carried out under conditions presumed to exist in vivo. These results indicate the complexity of lipid-protein interactions as regulators of membrane-bound enzymes. They also indicate the functional significance of this type of regulation for the activity of UDPglucuronyltransferase.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
410
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
61-73
pubmed:dateRevised
2009-10-27
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
Regulation of microsomal enzymes by phospholipids. IX. Production of uniquely modified forms of microsomal UDP-glucuronyltransferase by treatment with phospholipase A and detergents.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.