Linked Life Data

8109361

Source:http://linkedlifedata.com/resource/pubmed/id/8109361

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1994-3-21
pubmed:abstractText
Single fibers from glycerinated rabbit psoas muscle were treated with 1-ethyl-3[3-(dimethylamino) propyl] carbodiimide (EDC), after rigor was induced, to crosslink myosin heads to actin. The optimally pre-stretched (approximately 1.8%), partially crosslinked fibers produce a large force when MgATP is depleted, and this force is abolished when MgATP is reintroduced, even in high ionic strength solution of 0.5 M (Tawada et al. 1989). We investigated the rate of force decay in the crosslinked, force-producing fibers using pulse photolysis of caged ATP (Goldman et al. 1984). The decay of force was fast, the rate of which depending both on the ionic strength and on the amount of ATP released (0.2-2.2 mM) with the second-order rate constant of 0.5-1 x 10(5) M-1s-1 at the ionic strength of 0.5 M. At high ionic strength (1-2M) force decayed at lower rate. At low ionic strength (0.1-0.2 M), however, force decayed more rapidly, but force redeveloped subsequently, which is probably caused by uncrosslinked myosin heads.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0065-2598
pubmed:author
pubmed:issnType
Print
pubmed:volume
332
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
489-93; discussion 493-4
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Effects of ionic strength on force transients induced by flash photolysis of caged ATP in covalently crosslinked rabbit psoas muscle fibers.
pubmed:affiliation
Department of Physiology, Medical College of Oita, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't