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rdf:type |
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|
lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1994-10-5
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pubmed:abstractText |
We present NMR studies of an intramolecular triple helix, the three strands of which have been linked by a hexaethylene glycol chain. To overcome the generally encountered difficulties of assignment in the homo-pyrimidine strands, the carbon Cl' of the pyrimidines were selectively 13C-enriched. Assignments of the aromatic and sugar protons were obtained from NOESY-HMQC and TOCSY-HMQC spectra. We show that the recognition of a DNA duplex by a third strand via triplex formation is easily carried out in solution by observing the changes of the 1Hl'-13Cl' connectivities as a function of pH. Furthermore, the conformation of the sugars has been found to be C2'-endo, on the basis of the coupling constant values directly measured in an HSQC spectrum.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0925-2738
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
575-80
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
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pubmed:year |
1994
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|
pubmed:articleTitle |
Selectively 13C-enriched DNA: 13C and 1H assignments of a triple helix by two-dimensional relayed HMQC experiments.
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pubmed:affiliation |
Centre de Biophysique Moléculaire, CNRS, Orléans, France.
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|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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