| pubmed-article:8061224 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8061224 | lifeskim:mentions | umls-concept:C0806140 | lld:lifeskim |
| pubmed-article:8061224 | lifeskim:mentions | umls-concept:C1709845 | lld:lifeskim |
| pubmed-article:8061224 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8061224 | pubmed:dateCreated | 1994-9-16 | lld:pubmed |
| pubmed-article:8061224 | pubmed:abstractText | A new type of flow reactor (UCSTR) has been developed that uses anisotropic ultrafiltration membranes in a continuous flow stirred tank reactor (CSTR) to facilitate the study of nonlinear enzyme catalyzed reactions. The design allows the study of enzymes with subunit molecular weights > or = 9000 dalton and protein concentrations up to at least 2 mg/ml under flow conditions with a residence time of 3 min or more, in a reactor of volume 1.67 ml. The UCSTR allows continuous potentiometric or spectrophotometric measurement without design change. Calibration of reactor performance was carried out by reproducing pH oscillations in the ferrocyanide-hydrogen peroxide reaction. Experimental verification of oscillatory glycolysis in the UCSTR was carried out with extract of rat skeletal muscle. Input feeds were fructose-6-phosphate and ATP with low concentrations of phosphate as buffer. Oscillations in pH, sustained for over eight hours, were observed. A six-step mechanism, including product activation and substrate inhibition, seven concentration variables, and four enzymes sufficed simulate the pH oscillations observed in the UCSTR. | lld:pubmed |
| pubmed-article:8061224 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8061224 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8061224 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8061224 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8061224 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8061224 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8061224 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8061224 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:8061224 | pubmed:issn | 0301-4622 | lld:pubmed |
| pubmed-article:8061224 | pubmed:author | pubmed-author:KustinKK | lld:pubmed |
| pubmed-article:8061224 | pubmed:author | pubmed-author:TornheimKK | lld:pubmed |
| pubmed-article:8061224 | pubmed:author | pubmed-author:EpsteinI RIR | lld:pubmed |
| pubmed-article:8061224 | pubmed:author | pubmed-author:HockerC GCG | lld:pubmed |
| pubmed-article:8061224 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8061224 | pubmed:volume | 51 | lld:pubmed |
| pubmed-article:8061224 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8061224 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8061224 | pubmed:pagination | 21-35 | lld:pubmed |
| pubmed-article:8061224 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8061224 | pubmed:meshHeading | pubmed-meshheading:8061224-... | lld:pubmed |
| pubmed-article:8061224 | pubmed:meshHeading | pubmed-meshheading:8061224-... | lld:pubmed |
| pubmed-article:8061224 | pubmed:meshHeading | pubmed-meshheading:8061224-... | lld:pubmed |
| pubmed-article:8061224 | pubmed:meshHeading | pubmed-meshheading:8061224-... | lld:pubmed |
| pubmed-article:8061224 | pubmed:meshHeading | pubmed-meshheading:8061224-... | lld:pubmed |
| pubmed-article:8061224 | pubmed:meshHeading | pubmed-meshheading:8061224-... | lld:pubmed |
| pubmed-article:8061224 | pubmed:meshHeading | pubmed-meshheading:8061224-... | lld:pubmed |
| pubmed-article:8061224 | pubmed:meshHeading | pubmed-meshheading:8061224-... | lld:pubmed |
| pubmed-article:8061224 | pubmed:meshHeading | pubmed-meshheading:8061224-... | lld:pubmed |
| pubmed-article:8061224 | pubmed:meshHeading | pubmed-meshheading:8061224-... | lld:pubmed |
| pubmed-article:8061224 | pubmed:meshHeading | pubmed-meshheading:8061224-... | lld:pubmed |
| pubmed-article:8061224 | pubmed:meshHeading | pubmed-meshheading:8061224-... | lld:pubmed |
| pubmed-article:8061224 | pubmed:meshHeading | pubmed-meshheading:8061224-... | lld:pubmed |
| pubmed-article:8061224 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8061224 | pubmed:articleTitle | Glycolytic pH oscillations in a flow reactor. | lld:pubmed |
| pubmed-article:8061224 | pubmed:affiliation | Department of Chemistry, Brandeis University, Waltham, MA 02254-9110. | lld:pubmed |
| pubmed-article:8061224 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8061224 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8061224 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
