Linked Life Data

8060303

Source:http://linkedlifedata.com/resource/pubmed/id/8060303

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1994-9-12
pubmed:databankReference
pubmed:abstractText
Bovine monocyte chemoattractant protein-1 (bovine MCP-1) cDNA has recently been characterized and shown to be highly expressed in bovine seminal vesicles. In an attempt to isolate the MCP-1 gene, we screened a bovine genomic lambda EMBL-3 library with an MCP-1 specific probe pH42. A positive clone lambda MCP1 was subjected to restriction analysis and a pH42-positive EcoRI-fragment of 3.8 kb was subcloned and sequenced. The bovine MCP-1 gene consists of three exons separated by two introns. We undertook to identify homologous sequences 5'-upstream of the transcriptional start site within the genes of rat, mouse, human and bovine MCP-1. Six conserved sequence stretches located within 400 bp upstream of the transcriptional start site in MCP-1 genes were detected. Interestingly an approximately 45 bp long region, displaying identities in the range of 68-72% relative to the human sequence, covers in the rat gene the -141/88 region responsible for TPA induction of gene expression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
202
pubmed:geneSymbol
MCP-1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1272-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Characterization of the bovine monocyte chemoattractant protein-1 gene.
pubmed:affiliation
Dept. Molecular Biology, Max-Planck-Institute for Biophysical Chemistry, Göttingen, Germany.
pubmed:publicationType
Journal Article