Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1994-8-22
pubmed:abstractText
2-Deoxy-2-fluoro-beta-glucosyl and -beta-mannosyl fluorides administered to rats in a single dose (10 mg/kg) inhibited beta-glucosidase and beta-mannosidase activity respectively after 1 h in brain, spleen, liver and kidney tissues. This inhibition, presumably caused by accumulation of 2-deoxy-2-fluoroglycosyl-enzyme intermediates, indicates that intact 2-deoxy-2-fluoroglycosyl fluorides are distributed to these organs and, in the case of brain, that they cross the blood/brain barrier. beta-Glucosidase activity recovered completely or partially in brain, spleen, liver and kidney by 20-48 h. beta-Mannosidase activity partially recovered in all tissues by 48 h. beta-Galactosidase activity in brain and kidney was not significantly affected by administration of either the gluco or manno compounds at this dosage, indicating that these inhibitors are directed towards specific glycosidases. Observation of similar relatively rapid rates of beta-glycosidase re-activation in vivo and in tissue homogenates in vitro at 37 degrees C suggests that hydrolysis or transglycosylation of 2-deoxy-2-fluoroglycosyl-enzymes, not protein synthesis, are the primary mechanisms involved in the recovery of glycosidase activity inhibited by this class of compounds in vivo.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8042975-1239284, http://linkedlifedata.com/resource/pubmed/commentcorrection/8042975-1386724, http://linkedlifedata.com/resource/pubmed/commentcorrection/8042975-1390781, http://linkedlifedata.com/resource/pubmed/commentcorrection/8042975-14253443, http://linkedlifedata.com/resource/pubmed/commentcorrection/8042975-1995562, http://linkedlifedata.com/resource/pubmed/commentcorrection/8042975-2127241, http://linkedlifedata.com/resource/pubmed/commentcorrection/8042975-2304, http://linkedlifedata.com/resource/pubmed/commentcorrection/8042975-237551, http://linkedlifedata.com/resource/pubmed/commentcorrection/8042975-2945113, http://linkedlifedata.com/resource/pubmed/commentcorrection/8042975-2963589, http://linkedlifedata.com/resource/pubmed/commentcorrection/8042975-3124752, http://linkedlifedata.com/resource/pubmed/commentcorrection/8042975-3286645, http://linkedlifedata.com/resource/pubmed/commentcorrection/8042975-33720, http://linkedlifedata.com/resource/pubmed/commentcorrection/8042975-3929788, http://linkedlifedata.com/resource/pubmed/commentcorrection/8042975-4085458, http://linkedlifedata.com/resource/pubmed/commentcorrection/8042975-4236776, http://linkedlifedata.com/resource/pubmed/commentcorrection/8042975-5983454, http://linkedlifedata.com/resource/pubmed/commentcorrection/8042975-6772162, http://linkedlifedata.com/resource/pubmed/commentcorrection/8042975-7061483, http://linkedlifedata.com/resource/pubmed/commentcorrection/8042975-7228876, http://linkedlifedata.com/resource/pubmed/commentcorrection/8042975-958319
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
301 ( Pt 2)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
343-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
In vivo inhibition of beta-glucosidase and beta-mannosidase activity in rats by 2-deoxy-2-fluoro-beta-glycosyl fluorides and recovery of activity in vivo and in vitro.
pubmed:affiliation
Department of Chemistry, University of British Columbia, Vancouver, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't