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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1994-7-19
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pubmed:abstractText |
1. To investigate the role of long-term stimulation of nicotinic acetylcholine receptors (AChRs) on the regulation of membrane potential, non-contracting C2C12 myotubes were stimulated for 1-4 days with carbachol (10 microM) and membrane potentials were measured by the intracellular microelectrode technique after washing out of the drug. 2. The membrane potential (-45.7 mV) gradually increased by 10.1 mV to -55.8 mV during 4 days treatment, which was caused by enhanced electrogenic Na+/K(+)-pumping. 3. The concentration-dependent enhancement of Na+/K(+)-ATPase activity in long-term carbachol-treated myotubes (4 days, EC50 = 5.3 microM) was prevented by co-treatment with the competitive nicotinic AChR antagonist, pancuronium but not by the muscarinic antagonist, atropine. 4. Enhanced Na+/K(+)-ATPase activity still developed in carbachol-stimulated myotubes during co-treatment (4 days) with the nicotinic AChR-channel blocker, chlorpromazine (1 microM). Membrane depolarization as such, obtained by incubation in high K+ medium (40 mM, 4 days) did not enhance Na+/K(+)-ATPase activity. 5. Non-treated myotubes possessed a high-affinity ouabain binding site (Kd = 119 nM) in association with the low Na+/K(+)-pumping activity. Long-term stimulation of myotubes (4 days) with carbachol or with a combination of carbachol and chlorpromazine was accompanied by the development of an additional low-affinity ouabain binding site (Kd = 13 microM). 6. Binding of monoclonal antibodies directed against either alpha 1- or alpha 2-subunit of Na+/K(+)-ATPase were both increased in myotubes treated with carbachol (4 days). 7. These results support the concept that nicotinic AChRs regulate Na+/K(+)-ATPase activity, independent of the functionality of the receptor-operated ion-channel.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-1331053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-1393284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-1592722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-166159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-1689313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-170397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-2080554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-2176238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-2455720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-2541792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-2542271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-2579711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-3015915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-4171017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-5032352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-563524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-6254391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-6254793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-6373449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-64930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-722508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-839441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8004390-8469610
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0007-1188
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
111
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
459-64
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8004390-Animals,
pubmed-meshheading:8004390-Antibodies, Monoclonal,
pubmed-meshheading:8004390-Carbachol,
pubmed-meshheading:8004390-Cell Line,
pubmed-meshheading:8004390-Cholinergic Antagonists,
pubmed-meshheading:8004390-Electrophysiology,
pubmed-meshheading:8004390-Enzyme Induction,
pubmed-meshheading:8004390-Membrane Potentials,
pubmed-meshheading:8004390-Mice,
pubmed-meshheading:8004390-Microtubules,
pubmed-meshheading:8004390-Muscles,
pubmed-meshheading:8004390-Ouabain,
pubmed-meshheading:8004390-Receptors, Cholinergic,
pubmed-meshheading:8004390-Sodium-Potassium-Exchanging ATPase,
pubmed-meshheading:8004390-Up-Regulation
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pubmed:year |
1994
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pubmed:articleTitle |
Induction of Na+/K(+)-ATPase activity by long-term stimulation of nicotinic acetylcholine receptors in C2C12 myotubes.
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pubmed:affiliation |
Department of Pharmacology/Clinical Pharmacology, University of Groningen, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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