|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0001629,
umls-concept:C0007452,
umls-concept:C0007600,
umls-concept:C0017262,
umls-concept:C0185117,
umls-concept:C0205245,
umls-concept:C0259540,
umls-concept:C0597694,
umls-concept:C0815000,
umls-concept:C0936012,
umls-concept:C1522642,
umls-concept:C2911684
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1994-12-9
|
|
pubmed:abstractText |
Using a cDNA library from bovine adrenal medulla, and, subsequently, a bovine genomic library, we have isolated the gene coding for a non inactivating potassium channel. This gene encodes a 597-amino acid protein which we have called BAK5 as its sequence is very similar to members of Kv1.5 potassium channel family. Neuroblastoma cells (Neuro-2a cell line) were stably transfected with BAK5 DNA. Protein expression was under the control of a heat-shock promoter. Transfected cells showed a current highly selective for potassium, insensitive to tetraethylammonium but reversibly blocked by 4-aminopyridine. Oocytes injected with BAK5 mRNA also expressed a potassium current with the same characteristics.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0014-5793
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
7
|
|
pubmed:volume |
354
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
173-6
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:7957920-4-Aminopyridine,
pubmed-meshheading:7957920-Adrenal Medulla,
pubmed-meshheading:7957920-Amino Acid Sequence,
pubmed-meshheading:7957920-Animals,
pubmed-meshheading:7957920-Base Sequence,
pubmed-meshheading:7957920-Cattle,
pubmed-meshheading:7957920-Cloning, Molecular,
pubmed-meshheading:7957920-Female,
pubmed-meshheading:7957920-Gene Expression,
pubmed-meshheading:7957920-Humans,
pubmed-meshheading:7957920-Molecular Sequence Data,
pubmed-meshheading:7957920-Neuroblastoma,
pubmed-meshheading:7957920-Oocytes,
pubmed-meshheading:7957920-Potassium,
pubmed-meshheading:7957920-Potassium Channels,
pubmed-meshheading:7957920-RNA, Messenger,
pubmed-meshheading:7957920-Rats,
pubmed-meshheading:7957920-Tetraethylammonium,
pubmed-meshheading:7957920-Tetraethylammonium Compounds,
pubmed-meshheading:7957920-Transfection,
pubmed-meshheading:7957920-Tumor Cells, Cultured,
pubmed-meshheading:7957920-Xenopus
|
|
pubmed:year |
1994
|
|
pubmed:articleTitle |
A delayed rectifier potassium channel cloned from bovine adrenal medulla. Functional analysis after expression in Xenopus oocytes and in a neuroblastoma cell line.
|
|
pubmed:affiliation |
Department of Neurochemistry, Universidad de Alicante, Spain.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
