Linked Life Data

7957175

Source:http://linkedlifedata.com/resource/pubmed/id/7957175

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-11-28
pubmed:abstractText
Sin a I, the major allergen from mustard seeds, interacts with acid phospholipid vesicles. The protein binds to dimyristoylglycerophosphoglycerol vesicles with an apparent dissociation constant of approximately 2.4 microM, the number of phospholipid molecules affected by one protein molecule being approximately 20. Sin a I promotes an increase in the light scattering of a vesicle suspension. This process becomes saturated at approximately a lipid/protein molar ratio of 20:1. Sin a I also modifies the thermotropic behaviour of the negatively charged vesicles, which has been studied by measuring the fluorescence polarization of the probe 1,6-diphenyl-1,3,5-hexatriene incorporated into the hydrophobic core of the bilayer. Sin a I also promotes lipid mixing between vesicles. This mixing has been analyzed by measuring the variation of the fluorescence energy transfer between N-(7-nitro-2-1,3-benzoxadiazol-4-yl)-dimyristoylglycerophosphoe thanolamine (donor) and N-(lissamine rhodamine B sulphonyl)-PtdEtn (acceptor) incorporated into dimyristoylglycerophosphoglycerol vesicles. This effect is also corroborated by observing a single thermotropic transition in a mixture of independent dipalmitoylglycerophosphoglycerol and dimyristoylglycerophosphoglycerol vesicles when Sin a I is added to the lipid suspension. The allergen promotes release of aqueous contents of PtdGro vesicles, as determined by an aminonaphthalenetrisulfonic acid/p-xylylenebis(pyridinium)bromide dequenching assay. This study shows that the allergen Sin a I is able to interact with membrane lipids. This interaction is discussed in terms of its potential involvement in the allergenicity of this protein.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
225
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
609-15
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Food mustard allergen interaction with phospholipid vesicles.
pubmed:affiliation
Departamento de Bioquímica y Biología Molecular, Facultad de Química, Universidad Complutense, Madrid, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't