Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3 Pt 1
|
| pubmed:dateCreated |
1994-10-24
|
| pubmed:abstractText |
To evaluate the effects of physical training on mitochondrial gene expression and mitochondrial biogenesis in slow-twitch muscle, adult female Sprague-Dawley rats were trained for 3, 6, and 12 wk by running on a motor-driven treadmill (speed of 25 m/min and duration of 90 min/day, 5 days/wk), and the activities of citrate synthase, ubiquinol-cytochrome-c oxidoreductase, cytochrome oxidase, mitochondrial cytochrome b mRNA (by Northern blot analysis), and mitochondrial DNA (by slot-blot and Southern blot analyses) were measured in rat soleus muscle. A DNA probe for detection of mitochondrial mRNA and DNA was prepared from a 1,500-bp fragment of human mitochondrial DNA that included the coding region of the cytochrome b gene. Training for 3, 6, and 12 wk significantly increased the activities of citrate synthase (31, 28, and 47%, respectively), ubiquinol-cytochrome-c oxidoreductase (61, 63, and 77%, respectively), and cytochrome oxidase (25, 26, and 32%, respectively) in muscle. The concentration of cytochrome b mRNA in the muscle was proportionally elevated with the enzyme activities. On the other hand, the mitochondrial DNA concentration in the muscle was not altered by training for 3 or 6 wk but increased significantly after training for 12 wk (35% in the slot-blot analysis and 31% in the Southern blot analysis). These results suggest that an increase in the oxidative capacity of slow-twitch muscle by the relatively short-term training is regulated at the pretranslational step in mitochondrial protein synthesis but that the increase by the long-term training involves mitochondrial replication.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome b Group,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Mitochondrial,
http://linkedlifedata.com/resource/pubmed/chemical/Molecular Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Muscle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0002-9513
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
267
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
E388-95
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7943219-Adaptation, Physiological,
pubmed-meshheading:7943219-Animals,
pubmed-meshheading:7943219-Cytochrome b Group,
pubmed-meshheading:7943219-DNA,
pubmed-meshheading:7943219-DNA, Mitochondrial,
pubmed-meshheading:7943219-Female,
pubmed-meshheading:7943219-Gene Expression,
pubmed-meshheading:7943219-Hindlimb,
pubmed-meshheading:7943219-Microscopy, Electron,
pubmed-meshheading:7943219-Mitochondria, Muscle,
pubmed-meshheading:7943219-Molecular Probes,
pubmed-meshheading:7943219-Muscle Proteins,
pubmed-meshheading:7943219-Muscles,
pubmed-meshheading:7943219-Physical Conditioning, Animal,
pubmed-meshheading:7943219-Polymerase Chain Reaction,
pubmed-meshheading:7943219-RNA, Messenger,
pubmed-meshheading:7943219-Rats,
pubmed-meshheading:7943219-Rats, Sprague-Dawley,
pubmed-meshheading:7943219-Time Factors
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Enzymatic and genetic adaptation of soleus muscle mitochondria to physical training in rats.
|
| pubmed:affiliation |
Department of Bioscience, Nagoya Institute of Technology, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|