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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1995-3-1
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pubmed:abstractText |
The spontaneous locomotion of immunomagnetically isolated resting CD4+ and CD8+ lymphocytes in three-dimensional collagen gels was recorded by time-lapse videomicroscopy. Two-dimensional projections of the paths of randomly selected individual cells were digitized, plotted and quantitatively analysed. Among five different donors 46 +/- 10% of CD4+ and CD8+ lymphocytes (n = 180) showed initial spontaneous locomotion (individual speed 5-25 microns/min; mean speed CD4+ 5.2 +/- 3.6 microns, CD8+ 3.23 +/- 2.72 microns). Active CD4+ cells were constantly migrating for more than 4 hr, whereas CD8+ lymphocytes significantly slowed down after 60-90 min in the collagen gel (P < 0.003). Quantitative analysis of the paths indicated at least three migratory phenotypes: (1) spontaneously locomoting cells exhibiting high speed and low frequency of stopping; (2) a major non-motile fraction without significant displacement; and (3) a subpopulation within CD4+ and CD8+ cells with intermediate activity of speed and stopping. Further subtype analysis of immunomagnetically isolated CD45RAhigh/ROlow or CD45RAlow/ROhigh lymphocytes showed that more than 90% of CD4+ CD45RAhigh/ROlow cells were actively locomoting. In contrast, only 20% of the CD4+ CD45ROhigh/RAlow phenotype showed spontaneous motility to a limited degree. The data indicate that resting CD4+ and CD8+ lymphocytes comprise further locomotory subpopulation related to the expression of different CD45 isoforms.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-1371526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-1372018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-1372559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-1380034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-1385607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-1431096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-1590996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-1705667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-1717579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-1721021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-1826015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-1831615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-1860621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-1972721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-1978253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-2139099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-2307933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-2458942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-2544434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-2894392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-3654786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-7085756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-7678616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-7684415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-7901283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-8094710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-8436407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-8450205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7835926-8462784
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
617-24
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7835926-Antigens, CD45,
pubmed-meshheading:7835926-CD4-Positive T-Lymphocytes,
pubmed-meshheading:7835926-CD8-Positive T-Lymphocytes,
pubmed-meshheading:7835926-Cell Movement,
pubmed-meshheading:7835926-Cells, Cultured,
pubmed-meshheading:7835926-Collagen,
pubmed-meshheading:7835926-Extracellular Matrix,
pubmed-meshheading:7835926-Humans,
pubmed-meshheading:7835926-Kinetics,
pubmed-meshheading:7835926-Microscopy, Video,
pubmed-meshheading:7835926-T-Lymphocyte Subsets
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pubmed:year |
1994
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pubmed:articleTitle |
Locomotor phenotypes of unstimulated CD45RAhigh and CD45ROhigh CD4+ and CD8+ lymphocytes in three-dimensional collagen lattices.
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pubmed:affiliation |
Faculty of Dentistry, McGill University, Montreal, Quebec, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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