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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1995-7-19
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pubmed:abstractText |
Bacterial sex pheromone, cPD1, induces sexual aggregation of Enterococcus faecalis harboring the bacteriocin plasmid, pPD1, and enables pPD1 to transfer at high frequency in a liquid culture. PD78 is a cPD1-inducible cell surface protein encoded by pPD1. The PD78 gene, traF, was disrupted by homologous recombination between pPD1 and an artificial vector having a deletion in the middle portion of traF. The disruption of traF did not affect the cPD1-inducible aggregation but reduced the transfer frequency of pPD1 to 2% of the wild-type level.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
May
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pubmed:issn |
0378-1097
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
15
|
pubmed:volume |
128
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pubmed:geneSymbol |
traF
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
283-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7781976-Bacterial Proteins,
pubmed-meshheading:7781976-Bacteriocins,
pubmed-meshheading:7781976-Blotting, Southern,
pubmed-meshheading:7781976-Conjugation, Genetic,
pubmed-meshheading:7781976-Enterococcus faecalis,
pubmed-meshheading:7781976-Escherichia coli,
pubmed-meshheading:7781976-Genes, Bacterial,
pubmed-meshheading:7781976-Mutagenesis, Insertional,
pubmed-meshheading:7781976-Pheromones,
pubmed-meshheading:7781976-Plasmids,
pubmed-meshheading:7781976-Transformation, Genetic
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pubmed:year |
1995
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pubmed:articleTitle |
Targeted disruption of the PD78 gene (traF) reduces pheromone-inducible conjugal transfer of the bacteriocin plasmid pPD1 in Enterococcus faecalis.
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pubmed:affiliation |
Department of Applied Biological Chemistry, University of Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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