Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-7-10
pubmed:abstractText
The urea cycle takes place in the hepatocyte of ureothelic animals. The conversion of ammonia into urea involves five reactions. The first 2 take place in the matrix of the mitochondria, the last 2 occur in the cytosol. Argininosuccinate synthetase (AS) is the third reaction of the urea cycle. It catalyses the condensation of citrulline and aspartate into argininosuccinate. We have previously reported that rat AS activity was present in the cytosol and the outer membrane of the mitochondria. We have shown that, at the activity level, the colocation of AS was changing during fetal and neonatal development and was under the control of corticosteroid and pancreatic hormones. However, an unresolved issue was whether both AS had the same specific activity and that their location was changing during ontogenesis or that the specific activities of mitochondrial and cytosolic enzymes were different and/or modified during this period. In the present report, we compared the compartmentalization of AS activity and protein level in the fetus, the new-born and the adult rat and the role of corticosteroid and pancreatic hormones. Specific activities of both AS remained unchanged during ontogenesis. Glucocorticoids induced an increase in mitochondrial AS while glucagon appeared to induce a concomitant decrease in the level of mitochondrial AS and an increase in cytosolic AS.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0300-8177
pubmed:author
pubmed:issnType
Print
pubmed:day
9
pubmed:volume
143
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
47-51
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7776957-Adrenal Cortex Hormones, pubmed-meshheading:7776957-Adrenalectomy, pubmed-meshheading:7776957-Animals, pubmed-meshheading:7776957-Animals, Newborn, pubmed-meshheading:7776957-Argininosuccinate Synthase, pubmed-meshheading:7776957-Cells, Cultured, pubmed-meshheading:7776957-Cytoplasm, pubmed-meshheading:7776957-Dexamethasone, pubmed-meshheading:7776957-Diabetes Mellitus, Experimental, pubmed-meshheading:7776957-Female, pubmed-meshheading:7776957-Glucagon, pubmed-meshheading:7776957-Hydrocortisone, pubmed-meshheading:7776957-Hypophysectomy, pubmed-meshheading:7776957-Liver, pubmed-meshheading:7776957-Male, pubmed-meshheading:7776957-Mitochondria, Liver, pubmed-meshheading:7776957-Pancreatic Hormones, pubmed-meshheading:7776957-Pregnancy, pubmed-meshheading:7776957-Rats, pubmed-meshheading:7776957-Rats, Wistar
pubmed:year
1995
pubmed:articleTitle
Regulation of argininosuccinate synthetase level by corticosteroid and pancreatic hormones during perinatal period.
pubmed:affiliation
Université de Bourgogne, Unité de Recherche en Nutrition Cellulaire et Métabolique, Dijon, France.
pubmed:publicationType
Journal Article, Comparative Study