Linked Life Data

7757232

Source:http://linkedlifedata.com/resource/pubmed/id/7757232

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-6-26
pubmed:abstractText
Based on a detailed study of retention parameters, reversed-phase ion-pair chromatographic methods were developed for the simultaneous determination of dihydroxybenzoates, indicators of in-vivo hydroxyl free radical formation, transmitter amines and some metabolites to facilitate neurochemical investigations in rodent brain. Coupling of the separation methods with electrochemical detection and the use of short-chain perfluorinated carboxylic acids for ion-pairing, allowed for a fast and sensitive determination of salicylate-derived 2,3- and 2,5-dihydroxybenzoic acids and the major electroactive, hydroxylated aromatic compounds present in brain samples. Detection limits for the dihydroxybenzoates (signal-to-noise ratio = 2) were 18-22 fmol injected on the column. Basal levels of 2,3-dihydroxybenzoate and 2,5-dihydroxybenzoate in the the striatum of mice treated with salicylate were 72 +/- 13 and 94 +/- 11 ng/g wet tissue, respectively.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1572-6495
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
664
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
247-52
pubmed:dateRevised
2007-10-16
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Separation of dihydroxybenzoates, indicators of in-vivo hydroxyl free radical formation, in the presence of transmitter amines and some metabolites in rodent brain, using high-performance liquid chromatography with electrochemical detection.
pubmed:affiliation
Institute for Drug Research, Budapest, Hungary.
pubmed:publicationType
Journal Article