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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
13
|
| pubmed:dateCreated |
1995-5-10
|
| pubmed:abstractText |
The three fibrinogen genes belong to the class II hepatic acute phase proteins that are regulated in part by members of the interleukin-6 (IL-6) family of cytokines and glucocorticoids. The common DNA sequence that characterizes this group of proteins is a hexanucleotide CTGGGA residing in the promoter regions of these genes. Investigations of IL-6 control of the A alpha fibrinogen gene by electrophoretic mobility shift assays using a 30-base pair DNA probe containing the CTGGGA element revealed that a novel protein is associated with this site during non-IL-6-stimulated conditions. Sensitive time-course studies of IL-6 stimulation using primary hepatocyte cultures, high resolution polyacrylamide gel electrophoresis, and site-directed mutagenesis show that upon IL-6 stimulation of hepatocytes, this DNA binding protein transiently leaves the CTGGGA site and binds 12 base pairs down-stream but then begins to re-associate with the original DNA site at 1 h and is completed by 2 h. A recently characterized and cloned IL-6-activated transcription factor, Stat-3, which has been reported to bind a CT-GGGAA site in the alpha-2 macroglobulin gene, another member of the class II acute phase proteins, does not bind to the CTGGGA sequence in the A alpha fibrinogen gene. These findings reveal the presence of a previously undefined IL-6-regulated event, which involves a new DNA binding protein and demonstrates for the first time additional details of the kinetics of IL-6 control of fibrinogen gene expression.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA Probes,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fibrinogen,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
31
|
| pubmed:volume |
270
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
7580-6
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:7706306-Animals,
pubmed-meshheading:7706306-Base Sequence,
pubmed-meshheading:7706306-Binding Sites,
pubmed-meshheading:7706306-Cell Line,
pubmed-meshheading:7706306-Cell Nucleus,
pubmed-meshheading:7706306-Cells, Cultured,
pubmed-meshheading:7706306-DNA Probes,
pubmed-meshheading:7706306-DNA-Binding Proteins,
pubmed-meshheading:7706306-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7706306-Fibrinogen,
pubmed-meshheading:7706306-Inflammation,
pubmed-meshheading:7706306-Insulin,
pubmed-meshheading:7706306-Interleukin-6,
pubmed-meshheading:7706306-Lipopolysaccharides,
pubmed-meshheading:7706306-Liver,
pubmed-meshheading:7706306-Molecular Sequence Data,
pubmed-meshheading:7706306-Promoter Regions, Genetic,
pubmed-meshheading:7706306-Rats,
pubmed-meshheading:7706306-Rats, Sprague-Dawley,
pubmed-meshheading:7706306-Transcription Factors,
pubmed-meshheading:7706306-Transfection
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Detection of a novel transcription factor for the A alpha fibrinogen gene in response to interleukin-6.
|
| pubmed:affiliation |
Department of Cell Biology, University of Alabama at Birmingham 35294-0005, USA.
|
| pubmed:publicationType |
Journal Article
|