|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0003250,
umls-concept:C0003316,
umls-concept:C0006556,
umls-concept:C0007634,
umls-concept:C0017262,
umls-concept:C0081938,
umls-concept:C0086418,
umls-concept:C0205314,
umls-concept:C0205369,
umls-concept:C0292863,
umls-concept:C0439836,
umls-concept:C0679622,
umls-concept:C1171362,
umls-concept:C1334087,
umls-concept:C1444748,
umls-concept:C1515670,
umls-concept:C1711351,
umls-concept:C2611253
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
1993-6-21
|
|
pubmed:abstractText |
The monoclonal antibody 33B6 was found to be specific for the beta 1 integrin subunit. Treatment of leukocytes with this antibody induced a vigorous homotypic aggregation that had similar physiologic conditions as aggregation induced by a monoclonal antibody specific for the alpha 4 subunit. Expression of a beta 1 subunit on the cell surface was not sufficient for mAb 33B6-mediated aggregation to occur, since cells of the K562 erythroleukemia line failed to respond even though they expressed the beta 1 subunit and the 33B6 epitope. However, after transfection with cDNA encoding the alpha 4 subunit, K562 cells acquired the ability to aggregate in response to mAb 33B6 binding. By contrast, mAb 33B6 blocked cell binding to the endothelial surface protein vascular cell adhesion molecule-1 and the extracellular matrix protein fibronectin. These results suggest that the beta 1 epitope defined by mAb 33B6 may play a novel role in regulating leukocyte adhesive interactions.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Fibronectins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Integrin alpha4beta1,
http://linkedlifedata.com/resource/pubmed/chemical/Integrins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Cell Adhesion Molecule-1
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0730-2312
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
51
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
465-78
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:7684386-Animals,
pubmed-meshheading:7684386-Antibodies, Monoclonal,
pubmed-meshheading:7684386-Base Sequence,
pubmed-meshheading:7684386-Cell Adhesion Molecules,
pubmed-meshheading:7684386-Cell Aggregation,
pubmed-meshheading:7684386-Cell Line, Transformed,
pubmed-meshheading:7684386-DNA,
pubmed-meshheading:7684386-Epitopes,
pubmed-meshheading:7684386-Fibronectins,
pubmed-meshheading:7684386-Humans,
pubmed-meshheading:7684386-Immunoglobulin G,
pubmed-meshheading:7684386-Immunologic Deficiency Syndromes,
pubmed-meshheading:7684386-Integrin alpha4beta1,
pubmed-meshheading:7684386-Integrins,
pubmed-meshheading:7684386-Leukemia, Erythroblastic, Acute,
pubmed-meshheading:7684386-Leukocytes,
pubmed-meshheading:7684386-Mice,
pubmed-meshheading:7684386-Molecular Sequence Data,
pubmed-meshheading:7684386-Recombinant Fusion Proteins,
pubmed-meshheading:7684386-Transfection,
pubmed-meshheading:7684386-Tumor Cells, Cultured,
pubmed-meshheading:7684386-Vascular Cell Adhesion Molecule-1
|
|
pubmed:year |
1993
|
|
pubmed:articleTitle |
Homotypic leukocyte aggregation triggered by a monoclonal antibody specific for a novel epitope expressed by the integrin beta 1 subunit: conversion of nonresponsive cells by transfecting human integrin alpha 4 subunit cDNA.
|
|
pubmed:affiliation |
Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
