|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
1993-6-15
|
|
pubmed:abstractText |
A cDNA encoding the human platelet serotonin (5-HT) uptake site was isolated and sequenced using the PCR. The cDNA represents a approximately 3.1-kb mRNA transcript and contains an open reading frame encoding a hydrophobic polypeptide of 630 amino acids with 12 membrane-spanning segments, a calculated molecular mass of 70,320 Da, and an estimated isoelectrical point of 5.84. The human platelet 5-HT uptake site is identical with the human brain 5-HT transporter and approximately 92% homologous to the rat protein. Hydropathicity analysis indicates 12 membrane-spanning segments with two putative glycosylation sites within the second extracellular loop. The human platelet 5-HT uptake site contains two intraplasmatic consensus phosphorylation sites for cyclic AMP-dependent protein kinase recognition located in the cytoplasmatic N-terminal region and three potential protein kinase C phosphorylation sites. The identity of the human platelet 5-HT uptake site and the brain 5-HT transporter indicates that both proteins are encoded by the same single-copy gene, which has been assigned to the human chromosome 17. Our findings are likely to facilitate molecular pharmacologic and genetic investigations of the 5-HT transporter in psychiatric disorders.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0022-3042
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
60
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
2319-22
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:7684072-Adult,
pubmed-meshheading:7684072-Amino Acid Sequence,
pubmed-meshheading:7684072-Blood Platelets,
pubmed-meshheading:7684072-Brain,
pubmed-meshheading:7684072-Carrier Proteins,
pubmed-meshheading:7684072-Cerebral Cortex,
pubmed-meshheading:7684072-Chromosomes, Human, Pair 17,
pubmed-meshheading:7684072-Humans,
pubmed-meshheading:7684072-Membrane Glycoproteins,
pubmed-meshheading:7684072-Membrane Transport Proteins,
pubmed-meshheading:7684072-Nerve Tissue Proteins,
pubmed-meshheading:7684072-Oligodeoxyribonucleotides,
pubmed-meshheading:7684072-Polymerase Chain Reaction,
pubmed-meshheading:7684072-Protein Structure, Secondary,
pubmed-meshheading:7684072-RNA,
pubmed-meshheading:7684072-Raphe Nuclei,
pubmed-meshheading:7684072-Sequence Homology, Amino Acid,
pubmed-meshheading:7684072-Serotonin,
pubmed-meshheading:7684072-Serotonin Plasma Membrane Transport Proteins
|
|
pubmed:year |
1993
|
|
pubmed:articleTitle |
Primary structure of the human platelet serotonin uptake site: identity with the brain serotonin transporter.
|
|
pubmed:affiliation |
Department of Psychiatry, University of Würzburg, F.R.G.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study
|
