|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
5
|
|
pubmed:dateCreated |
1995-10-3
|
|
pubmed:abstractText |
To determine the specificity of signal transducer and activator of transcription (STAT) protein activation by box 3 motif-deficient hematopoietin receptors, expression vectors encoding the receptors for growth hormone, interleukin-3 (IL-3), and IL-4 were transiently transfected into COS-1 cells, together with expression vectors for Janus kinases (JAKs) and STAT proteins. Each receptor mediated a dose-dependent activation of STAT1 and STAT3, and for IL-3R and GHR this process was enhanced by JAK2. The data suggest that a box 3 motif in the cytoplasmic domain of the signal-transducing receptor to the JAK/STAT pathway. Transfection of the receptors, in combination with STAT3, into HepG2 cells reconstituted a cytokine-dependent stimulation of gene transcription through IL-6 response elements, providing evidence for a functional role of STAT3 in controlling gene expression.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/JAK2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Janus Kinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Colony-Stimulating Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-3,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Somatotropin,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0006-4971
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
86
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1671-9
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:7654999-Animals,
pubmed-meshheading:7654999-Carcinoma, Hepatocellular,
pubmed-meshheading:7654999-Cell Line,
pubmed-meshheading:7654999-Cercopithecus aethiops,
pubmed-meshheading:7654999-Cytokines,
pubmed-meshheading:7654999-DNA-Binding Proteins,
pubmed-meshheading:7654999-Enzyme Activation,
pubmed-meshheading:7654999-Gene Expression,
pubmed-meshheading:7654999-Humans,
pubmed-meshheading:7654999-Interleukin-6,
pubmed-meshheading:7654999-Janus Kinase 2,
pubmed-meshheading:7654999-Kinetics,
pubmed-meshheading:7654999-Liver Neoplasms,
pubmed-meshheading:7654999-Protein-Tyrosine Kinases,
pubmed-meshheading:7654999-Proto-Oncogene Proteins,
pubmed-meshheading:7654999-Receptors, Colony-Stimulating Factor,
pubmed-meshheading:7654999-Receptors, Interleukin,
pubmed-meshheading:7654999-Receptors, Interleukin-3,
pubmed-meshheading:7654999-Receptors, Interleukin-4,
pubmed-meshheading:7654999-Receptors, Somatotropin,
pubmed-meshheading:7654999-Recombinant Proteins,
pubmed-meshheading:7654999-STAT3 Transcription Factor,
pubmed-meshheading:7654999-Signal Transduction,
pubmed-meshheading:7654999-Trans-Activators,
pubmed-meshheading:7654999-Transcription, Genetic,
pubmed-meshheading:7654999-Transfection,
pubmed-meshheading:7654999-Tumor Cells, Cultured
|
|
pubmed:year |
1995
|
|
pubmed:articleTitle |
Receptors for interleukin-3 (IL-3) and growth hormone mediate an IL-6-type transcriptional induction in the presence of JAK2 or STAT3.
|
|
pubmed:affiliation |
Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
