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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
17
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pubmed:dateCreated |
1995-9-21
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pubmed:abstractText |
An automated oligonucleotide synthesizer has been developed that can simultaneously and rapidly synthesize up to 96 different oligonucleotides in a 96-well microtiter format using phosphoramidite synthesis chemistry. A modified 96-well plate is positioned under reagent valve banks, and appropriate reagents are delivered into individual wells containing the growing oligonucleotide chain, which is bound to a solid support. Each well has a filter bottom that enables the removal of spent reagents while retaining the solid support matrix. A seal design is employed to control synthesis environment and the entire instrument is automated via computer control. Synthesis cycle times for 96 couplings are < 11 min, allowing a plate of 96 20-mers to be synthesized in < 5 hr. Oligonucleotide synthesis quality is comparable to commercial machines, with average coupling efficiencies routinely > 98% across the entire 96-well plate. No significant well-to-well variations in synthesis quality have been observed in > 6000 oligonucleotides synthesized to date. The reduced reagent usage and increased capacity allow the overall synthesis cost to drop by at least a factor of 10. With the development of this instrument, it is now practical and cost-effective to synthesize thousands to tens of thousands of oligonucleotides.
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pubmed:grant | |
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7644513-1353738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7644513-1439769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7644513-1979162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7644513-3010776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7644513-8197459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7644513-8341614
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amides,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Indicators and Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoric Acids,
http://linkedlifedata.com/resource/pubmed/chemical/phosphoramidic acid
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
92
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7912-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7644513-Amides,
pubmed-meshheading:7644513-Automation,
pubmed-meshheading:7644513-Chromatography, High Pressure Liquid,
pubmed-meshheading:7644513-Computers,
pubmed-meshheading:7644513-Costs and Cost Analysis,
pubmed-meshheading:7644513-DNA,
pubmed-meshheading:7644513-Indicators and Reagents,
pubmed-meshheading:7644513-Oligodeoxyribonucleotides,
pubmed-meshheading:7644513-Phosphoric Acids
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pubmed:year |
1995
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pubmed:articleTitle |
An automated multiplex oligonucleotide synthesizer: development of high-throughput, low-cost DNA synthesis.
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pubmed:affiliation |
Department of Genetics, Stanford University School of Medicine, CA 94305, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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