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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1995-8-17
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pubmed:abstractText |
Our earlier studies have shown that mammalian pancreatic group I phospholipase A2 (PLA2-I) has its specific receptor (PLA2 receptor) on a wide range of mammalian cells and that the receptor-binding capability of PLA2-I is a property of this molecule separable from its enzymatic activity. To clarify whether PLA2 activity is required for eliciting a biological response via the receptor or not, we examined the enzymatic activity of PLA2-I/PLA2 receptor complex and the inducibility of prostaglandin (PG) E2 production in rat mesangial cells by mutant PLA2s-I. Using a recombinant soluble PLA2 receptor, we first found that PLA2-I could not hydrolyze a phospholipid substrate when complexed with the receptor. In the next experiment using various mutant porcine PLA2s-I, we found that PGE2 production in rat mesangial cells could be induced by a mutant PLA2-I which retained the receptor-binding activity but had almost completely lost its enzymatic activity. These findings indicate that the enzyme action of PLA2-I is not required for a PLA2-I-induced biological response, i.e., the augmentation of PGE2 production in rat mesangial cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-924X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
117
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
420-4
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pubmed:dateRevised |
2007-12-19
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pubmed:meshHeading |
pubmed-meshheading:7608133-Animals,
pubmed-meshheading:7608133-Binding, Competitive,
pubmed-meshheading:7608133-CHO Cells,
pubmed-meshheading:7608133-Cricetinae,
pubmed-meshheading:7608133-Dinoprostone,
pubmed-meshheading:7608133-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7608133-Glomerular Mesangium,
pubmed-meshheading:7608133-Kinetics,
pubmed-meshheading:7608133-Mutagenesis, Site-Directed,
pubmed-meshheading:7608133-Pancreas,
pubmed-meshheading:7608133-Phospholipases A,
pubmed-meshheading:7608133-Phospholipases A2,
pubmed-meshheading:7608133-Point Mutation,
pubmed-meshheading:7608133-Rats,
pubmed-meshheading:7608133-Receptors, Cell Surface,
pubmed-meshheading:7608133-Receptors, Phospholipase A2,
pubmed-meshheading:7608133-Recombinant Proteins,
pubmed-meshheading:7608133-Swine,
pubmed-meshheading:7608133-Transfection
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pubmed:year |
1995
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pubmed:articleTitle |
Pancreatic-type phospholipase A2 activates prostaglandin E2 production in rat mesangial cells by receptor binding reaction.
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pubmed:affiliation |
Shionogi Research Laboratories, Shionogi & Company, Ltd., Osaka.
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pubmed:publicationType |
Journal Article,
Comparative Study
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