Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1995-11-13
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28820, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28821, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28822, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28823, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28824, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28825, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28826, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28827, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28828, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28829, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28830, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28831, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28832, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28833, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28834, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28835, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28836, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28837, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28838, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28839, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28840, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28841, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28842, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28843, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28844, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28845, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28846, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28847, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28848, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D28849
pubmed:abstractText
We examined 24 C-type hepatitis specimens from Thailand and detected hepatitis C virus (HCV) RNA in all of them by RT-nested PCR for a portion of the HCV 5' non-coding (5' NC) region and a portion of the HCV core region. However, we failed to detect HCV RNA in 11 specimens by RT-nested PCR for a portion in the non-structural protein 5 (NS5) region that has been used commonly for HCV genotyping. We designed a new primer set for a separate portion of the NS5 region. Using this primer set, we succeeded in amplifying this portion in all 24 specimens. Two novel HCV genotypes, tentatively designated HCV-VII and HCV-VIII, were identified by sequencing these amplified regions. Our newly designed primers for RT-nested PCR may be useful for diagnosing infection as well as for genotyping unidentified HCV genomes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-1317
pubmed:author
pubmed:issnType
Print
pubmed:volume
76 ( Pt 9)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2323-7
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Novel genotypes of hepatitis C virus in Thailand.
pubmed:affiliation
Virology Division, National Cancer Center Research Institute, Tokyo, Japan.
pubmed:publicationType
Journal Article