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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
1996-1-17
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pubmed:abstractText |
In virtually all of the 200 group I introns sequenced thus far, the specificity of 5' splice-site cleavage is determined by a basepair between a uracil base at the end of the 5' exon and a guanine in an intron guide sequence which pairs with the nucleotides flanking the splice-site. It has been reported that two introns in the cytochrome oxidase subunit I gene of Aspergillus nidulans and Podospora anserina are exceptions to this rule and have a C.G basepair in this position. We have confirmed the initial reports and shown for one of them that RNA editing does not convert the C to a U. Both introns autocatalytically cleave the 5' splice-site. Mutation of the C to U in one intron reduces the requirement for Mg2+ and leads to an increase in the rate of cleavage. As the C base encodes a highly conserved amino acid, we propose that it is selected post-translationally at the level of protein function, despite its inferior splicing activity.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501471-1455230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501471-1736996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501471-1959129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501471-2088173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501471-2342465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501471-2549662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501471-2555264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501471-2558809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501471-2673931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501471-2678103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501471-2779562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501471-3043183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501471-6092056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501471-6092057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501471-6757759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501471-6817818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501471-7504953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501471-7839142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501471-7885828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501471-7926724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501471-8422962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7501471-8422976
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4466-70
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7501471-Ascomycota,
pubmed-meshheading:7501471-Aspergillus nidulans,
pubmed-meshheading:7501471-Base Composition,
pubmed-meshheading:7501471-Base Sequence,
pubmed-meshheading:7501471-Cloning, Molecular,
pubmed-meshheading:7501471-Conserved Sequence,
pubmed-meshheading:7501471-Cytidine,
pubmed-meshheading:7501471-DNA Primers,
pubmed-meshheading:7501471-Electron Transport Complex IV,
pubmed-meshheading:7501471-Fungi,
pubmed-meshheading:7501471-Introns,
pubmed-meshheading:7501471-Molecular Sequence Data,
pubmed-meshheading:7501471-Mutagenesis,
pubmed-meshheading:7501471-Polymerase Chain Reaction,
pubmed-meshheading:7501471-Protein Processing, Post-Translational,
pubmed-meshheading:7501471-RNA Splicing,
pubmed-meshheading:7501471-Selection, Genetic,
pubmed-meshheading:7501471-Uridine
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pubmed:year |
1995
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pubmed:articleTitle |
Two group I introns with a C.G basepair at the 5' splice-site instead of the very highly conserved U.G basepair: is selection post-translational?
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pubmed:affiliation |
Department of Biology, Temple University, Philadelphia, PA 19122, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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