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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
46
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pubmed:dateCreated |
1996-1-17
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pubmed:abstractText |
We investigated the molecular basis of the synergistic induction by interferon-gamma (IFN-gamma)/tumor necrosis factor-alpha (TNF-alpha) of human interleukin-6 (IL-6) gene in THP-1 monocytic cells, and compared it with the basis of this induction by lipopolysaccharide (LPS). Functional studies with IL-6 promoter demonstrated that three regions are the targets of the IFN-gamma and/or TNF-alpha action, whereas only one of these regions seemed to be implicated in LPS activation. The three regions concerned are: 1) a region between -73 and -36, which is the minimal element inducible by LPS or TNF-alpha; 2) an element located between -181 and -73, which appeared to regulate the response to IFN-gamma and TNF-alpha negatively; and 3) a distal element upstream of -224, which was inducible by IFN-gamma alone. LPS signaling was found to involve NF kappa B activation by the p50/p65 heterodimers. Synergistic induction of the IL-6 gene by IFN-gamma and TNF-alpha, in monocytic cells, involved cooperation between the IRF-1 and NF kappa B p65 homodimers with concomitant removal of the negative effect of the retinoblastoma control element present in the IL-6 promoter. This removal occurred by activation of the constitutive Sp1 factor, whose increased binding activity and phosphorylation were mediated by IFN-gamma.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Probes,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/IRF1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon Regulatory Factor-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sp1 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
270
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
27920-31
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:7499267-Base Sequence,
pubmed-meshheading:7499267-Cell Line,
pubmed-meshheading:7499267-Cell Nucleus,
pubmed-meshheading:7499267-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:7499267-DNA Probes,
pubmed-meshheading:7499267-DNA-Binding Proteins,
pubmed-meshheading:7499267-Escherichia coli,
pubmed-meshheading:7499267-Gene Expression,
pubmed-meshheading:7499267-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:7499267-Humans,
pubmed-meshheading:7499267-Interferon Regulatory Factor-1,
pubmed-meshheading:7499267-Interferon-gamma,
pubmed-meshheading:7499267-Interleukin-6,
pubmed-meshheading:7499267-Kinetics,
pubmed-meshheading:7499267-Lipopolysaccharides,
pubmed-meshheading:7499267-Molecular Sequence Data,
pubmed-meshheading:7499267-Monocytes,
pubmed-meshheading:7499267-NF-kappa B,
pubmed-meshheading:7499267-Oligodeoxyribonucleotides,
pubmed-meshheading:7499267-Phosphoproteins,
pubmed-meshheading:7499267-Promoter Regions, Genetic,
pubmed-meshheading:7499267-Recombinant Proteins,
pubmed-meshheading:7499267-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:7499267-Restriction Mapping,
pubmed-meshheading:7499267-Sp1 Transcription Factor,
pubmed-meshheading:7499267-Substrate Specificity,
pubmed-meshheading:7499267-Transcription Factors,
pubmed-meshheading:7499267-Transfection,
pubmed-meshheading:7499267-Tumor Necrosis Factor-alpha
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pubmed:year |
1995
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pubmed:articleTitle |
Triggering of the human interleukin-6 gene by interferon-gamma and tumor necrosis factor-alpha in monocytic cells involves cooperation between interferon regulatory factor-1, NF kappa B, and Sp1 transcription factors.
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pubmed:affiliation |
INSERM, U365, Interferons et Cytokines, Institut Curie, Section de Recherches, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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