| pubmed-article:7493044 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7493044 | lifeskim:mentions | umls-concept:C0038836 | lld:lifeskim |
| pubmed-article:7493044 | lifeskim:mentions | umls-concept:C0443286 | lld:lifeskim |
| pubmed-article:7493044 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:7493044 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:7493044 | pubmed:dateCreated | 1996-1-11 | lld:pubmed |
| pubmed-article:7493044 | pubmed:abstractText | Recent studies have demonstrated that nitric oxide (NO) in the presence of superoxide (O2-) may mediate mutagenesis via the N-nitrosation of DNA bases followed by nitrosative deamination to yield their hydroxylated derivatives. We have found that phorbol myristate acetate (PMA)-activated extravasated rat neutrophils (PMNs) will N-nitrosate 2,3-diaminonaphthalene (DAN) to yield its highly fluorescent nitrosation product 2,3-naphthotriazole (triazole) via the L-arginine dependent formation of NO. Addition of SOD enhanced triazole formation suggesting that O2- production may inhibit the N-nitrosating activity and thus the mutagenic activity of inflammatory PMNs. The objective of this study was to assess the role of superoxide as a modulator of NO-dependent N-nitrosation reactions using PMA-activated PMNs as well as a chemically defined-system that generates both NO and superoxide. We found that PMA-activation of PMNs reduced found that PMA-activation of PMNs reduced the amount of N-nitrosation of DAN by approximately 64% when compared to non-stimulated cells (450 vs. 1250 nM). Addition of SOD but not inactivated SOD or catalase to PMA-activated PMNs enhanced the formation of triazole by approximately 4-fold (1950 nM). In addition, we found that the NO-releasing spermine/NO adduct (Sp/NO; 50 microM) which produces approximately 1.0 nmol NO/min generated approximately 8000 nM of triazole whereas the combination of Sp/NO and a superoxide generator (hypoxanthine/xanthine oxidase) that produces approximately 1.0 nmol O2-/min reduced triazole formation by 90% (790 nM). Addition of SOD but not catalase restored the N-nitrosating activity. We conclude that equimolar fluxes of superoxide react rapidly with NO to generate products that have only limited ability to N-nitrosate aromatic amino compounds and thus may have limited ability to promote mutagenesis via the nitrosative deamination of DNA bases. | lld:pubmed |
| pubmed-article:7493044 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7493044 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7493044 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7493044 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7493044 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7493044 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7493044 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7493044 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7493044 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7493044 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7493044 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7493044 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7493044 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:7493044 | pubmed:issn | 1071-5762 | lld:pubmed |
| pubmed-article:7493044 | pubmed:author | pubmed-author:GibsonM FMF | lld:pubmed |
| pubmed-article:7493044 | pubmed:author | pubmed-author:GrishamM BMB | lld:pubmed |
| pubmed-article:7493044 | pubmed:author | pubmed-author:MilesA MAM | lld:pubmed |
| pubmed-article:7493044 | pubmed:author | pubmed-author:CookJ CJC | lld:pubmed |
| pubmed-article:7493044 | pubmed:author | pubmed-author:WinkDD | lld:pubmed |
| pubmed-article:7493044 | pubmed:author | pubmed-author:PacellaJJ | lld:pubmed |
| pubmed-article:7493044 | pubmed:author | pubmed-author:KirshinaMM | lld:pubmed |
| pubmed-article:7493044 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7493044 | pubmed:volume | 23 | lld:pubmed |
| pubmed-article:7493044 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7493044 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7493044 | pubmed:pagination | 379-90 | lld:pubmed |
| pubmed-article:7493044 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:7493044 | pubmed:meshHeading | pubmed-meshheading:7493044-... | lld:pubmed |
| pubmed-article:7493044 | pubmed:meshHeading | pubmed-meshheading:7493044-... | lld:pubmed |
| pubmed-article:7493044 | pubmed:meshHeading | pubmed-meshheading:7493044-... | lld:pubmed |
| pubmed-article:7493044 | pubmed:meshHeading | pubmed-meshheading:7493044-... | lld:pubmed |
| pubmed-article:7493044 | pubmed:meshHeading | pubmed-meshheading:7493044-... | lld:pubmed |
| pubmed-article:7493044 | pubmed:meshHeading | pubmed-meshheading:7493044-... | lld:pubmed |
| pubmed-article:7493044 | pubmed:meshHeading | pubmed-meshheading:7493044-... | lld:pubmed |
| pubmed-article:7493044 | pubmed:meshHeading | pubmed-meshheading:7493044-... | lld:pubmed |
| pubmed-article:7493044 | pubmed:meshHeading | pubmed-meshheading:7493044-... | lld:pubmed |
| pubmed-article:7493044 | pubmed:meshHeading | pubmed-meshheading:7493044-... | lld:pubmed |
| pubmed-article:7493044 | pubmed:meshHeading | pubmed-meshheading:7493044-... | lld:pubmed |
| pubmed-article:7493044 | pubmed:meshHeading | pubmed-meshheading:7493044-... | lld:pubmed |
| pubmed-article:7493044 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7493044 | pubmed:articleTitle | Effects of superoxide on nitric oxide-dependent N-nitrosation reactions. | lld:pubmed |
| pubmed-article:7493044 | pubmed:affiliation | Department of Physiology and Biophysics Louisiana State University Medical Center Shreveport 71130, USA. | lld:pubmed |
| pubmed-article:7493044 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7493044 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7493044 | lld:pubmed |
