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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1981-1-16
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pubmed:abstractText |
Four serological assays for detection of antibody reactivity against surface antigens of benign and malignant cells have been evaluated. In all four assays, antibody attached to the surface of a target cell is detected by a rosette of indicator red blood cells. Of the four assays (antibody mixed hemadsorption assay, immune adherence assay, anti-C3 mixed hemadsorption assay and protein A assay), the anti-C3 mixed hemadsorption assay showed highest sensitivity. By using purified IgM and IgG fractions we could demonstrate that, except for the protein A assay, all assays show reactivity both with IgG and IgM. Protein A assay detected only the IgG fraction, with high sensitivity. The optimization of the four assays is described and the importance of using multiple assays is emphasized.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0022-1759
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
37
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
71-81
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7430662-Antigen-Antibody Reactions,
pubmed-meshheading:7430662-Antigens, Surface,
pubmed-meshheading:7430662-Astrocytoma,
pubmed-meshheading:7430662-Cells, Cultured,
pubmed-meshheading:7430662-Complement C3,
pubmed-meshheading:7430662-Hemadsorption,
pubmed-meshheading:7430662-Humans,
pubmed-meshheading:7430662-Immune Adherence Reaction,
pubmed-meshheading:7430662-Immunoglobulins,
pubmed-meshheading:7430662-Male,
pubmed-meshheading:7430662-Rosette Formation,
pubmed-meshheading:7430662-Staphylococcal Protein A,
pubmed-meshheading:7430662-Temperature
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pubmed:year |
1980
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pubmed:articleTitle |
Comparison of multiple rosetting assays for detecting antibody reactivity of different immunoglobulin classes against surface antigens of benign and malignant tissue culture cells.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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