Linked Life Data

7379552

Source:http://linkedlifedata.com/resource/pubmed/id/7379552

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1980-8-15
pubmed:abstractText
Cultures of primary canine monocytes were prepared from blood to which dextran (3%) had been added to enhance sedimentation of the erythrocytes. Homogeneous populations of monocytes were observed by 5 days after seeding, and some cultures were maintained as long as 30 days. The monocyte cultures were used successfully for the serial passage of Ehrlichia canis. After the 3rd passage, small intracytoplasmic inclusions of E canis were detected by 60 hrs after inoculation and greater than 90% of the monocytes were infected by 7 days. In vitro propagated ehrlichiae were preserved at -80 C for at least 31 months with minimal loss of infectivity. Following 125 in vitro passages E canis induced signs of ehrlichiosis in experimentally infected dogs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0010-8901
pubmed:author
pubmed:issnType
Print
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
36-42
pubmed:dateRevised
2009-11-11
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Serial propagation of Ehrlichia canis in primary canine peripheral blood monocyte cultures.
pubmed:publicationType
Journal Article