Linked Life Data

7374655

Source:http://linkedlifedata.com/resource/pubmed/id/7374655

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1980-7-12
pubmed:abstractText
Undegraded Vicia faba polysomes from meristematic root cells were obtained after homogenization in a medium of low ionic strength provided that the pH was equal to 9.0. By minimizing the shearing forces during the homogenization step, polysomes were obtained free of mitochondrial and nuclear contaminants, measured by differential spectrophotometry and CsCl gradient centrifugation respectively. Poly(A)-containing RNA was obtained by poly(U)-Sepharose chromatography and shown to be virtually free of rRNA and its average size was 13-15 S. Approximately 9% of the purified preparation was annealed by [3H]-poly(U). Sucrose gradient analysis under denaturing conditions showed that the poly(A)-CONtaining RNA were non-degraded. This RNA was used to direct the synthesis of proteins in a heterologous cell-free system from wheat germ.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0300-8177
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
173-81
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Isolation and characterization of polysomes and polyadenylated polysomal RNA from Vicia faba meristematic root cells.
pubmed:publicationType
Journal Article