| pubmed-article:7287706 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7287706 | lifeskim:mentions | umls-concept:C0036866 | lld:lifeskim |
| pubmed-article:7287706 | lifeskim:mentions | umls-concept:C1882726 | lld:lifeskim |
| pubmed-article:7287706 | lifeskim:mentions | umls-concept:C0022702 | lld:lifeskim |
| pubmed-article:7287706 | lifeskim:mentions | umls-concept:C0439830 | lld:lifeskim |
| pubmed-article:7287706 | lifeskim:mentions | umls-concept:C1979928 | lld:lifeskim |
| pubmed-article:7287706 | lifeskim:mentions | umls-concept:C0205265 | lld:lifeskim |
| pubmed-article:7287706 | lifeskim:mentions | umls-concept:C0348080 | lld:lifeskim |
| pubmed-article:7287706 | lifeskim:mentions | umls-concept:C1555582 | lld:lifeskim |
| pubmed-article:7287706 | lifeskim:mentions | umls-concept:C0332120 | lld:lifeskim |
| pubmed-article:7287706 | lifeskim:mentions | umls-concept:C0059655 | lld:lifeskim |
| pubmed-article:7287706 | pubmed:issue | 20 | lld:pubmed |
| pubmed-article:7287706 | pubmed:dateCreated | 1981-12-15 | lld:pubmed |
| pubmed-article:7287706 | pubmed:abstractText | The enzyme kinetics of estrogen 2-hydroxylase from rat liver microsomes has been investigated using two radiotracer methods. The conversion of [4-14C]estradiol to [4-14C]2-hydroxyestradiol by microsomes was examined by isolation of the catechol estrogen. [6,7-3H]2-Hydroxyestradiol was prepared and added at the end of the incubation as a recovery marker for quantitation. Initial velocity conditions of less than 10% product formation were established. The enzyme kinetics for estrogen 2-hydroxylase from male rat liver follows classical Michaelis-Menten kinetics producing a linear Lineweaver-Burk plot. The apparent Km for estradiol under these conditions was 2.2 microM; the Vmax for freshly prepared microsomes was 5.0 nmol/mg/min while that for microsomes stored at -70 degrees C was 0.51 nmol/mg/min. On the other hand, estrogen 2-hydroxylase from female rat liver microsomes did not follow Michaelis-Menten kinetics and yielded a nonlinear double reciprocal plot. Analogous kinetic data were obtained with another radiotracer assay measuring the release of 3H2O from [2-3H]estradiol. Thus, the estrogen 2-hydroxylases of male and female rat liver differ in enzyme kinetic properties. The upwardly curving Lineweaver-Burk plot of the kinetic data of the female rat liver microsomes suggests that more than one binding site for estradiol may exist on this enzyme complex and/or that this particular estrogen 2-hydroxylase system exhibits cooperative effects. | lld:pubmed |
| pubmed-article:7287706 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7287706 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7287706 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7287706 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7287706 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7287706 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7287706 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7287706 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7287706 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7287706 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7287706 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7287706 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:7287706 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:7287706 | pubmed:author | pubmed-author:BrueggemeierR... | lld:pubmed |
| pubmed-article:7287706 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7287706 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:7287706 | pubmed:volume | 256 | lld:pubmed |
| pubmed-article:7287706 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7287706 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7287706 | pubmed:pagination | 10239-42 | lld:pubmed |
| pubmed-article:7287706 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:7287706 | pubmed:meshHeading | pubmed-meshheading:7287706-... | lld:pubmed |
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| pubmed-article:7287706 | pubmed:meshHeading | pubmed-meshheading:7287706-... | lld:pubmed |
| pubmed-article:7287706 | pubmed:meshHeading | pubmed-meshheading:7287706-... | lld:pubmed |
| pubmed-article:7287706 | pubmed:meshHeading | pubmed-meshheading:7287706-... | lld:pubmed |
| pubmed-article:7287706 | pubmed:meshHeading | pubmed-meshheading:7287706-... | lld:pubmed |
| pubmed-article:7287706 | pubmed:meshHeading | pubmed-meshheading:7287706-... | lld:pubmed |
| pubmed-article:7287706 | pubmed:year | 1981 | lld:pubmed |
| pubmed-article:7287706 | pubmed:articleTitle | Kinetics of rat liver microsomal estrogen 2-hydroxylase. Evidence for sex differences at initial velocity conditions. | lld:pubmed |
| pubmed-article:7287706 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7287706 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7287706 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7287706 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7287706 | lld:pubmed |
