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Class specific F(ab')2 antibody fragments were prepared by pepsin digestion of the labile Fc immunoglobulin fragments in antigen--antibody precipitates. The F(ab')2 fragments were covalently coupled to cyanogen bromide-activated Sepharose 4B and the resultant immunoadsorbent used to isolate IgG from human serum with a single chromatographic step, in high yield and purity and negligible non-specific interaction. This technique affords a simple method for preparing an enriched source of class specific affinity-purified immunoglobulin antibodies suitable for many immunochemical applications.
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