Linked Life Data

7256205

Source:http://linkedlifedata.com/resource/pubmed/id/7256205

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1981-9-25
pubmed:abstractText
The principles of x-ray microanalytical histochemistry are reviewed. The use of labelling and precipitation techniques are examined, and particular attention is paid to the localization of enzymatic activity. A new method is described for the simultaneous assessment of mitosis as represented by the incorporation of (3H) thymidine, and cell death as represented by the localization of free acid phosphatase, in the same tissue section. The thymidine incorporation is demonstrated by the appearance of topographically and microanalytically detectable silver grains in an overlying emulsion and the cell lysis associated acid phosphatase activity is detected optically and microanalytically by means of a bromine-rich azo dye deposited as a result of coupling naphthol AS BI, enzymatically released from naphthyl AS BI phosphoric acid, with diazotized 2,5-dibromoaniline.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0586-5581
pubmed:author
pubmed:issnType
Print
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
179-88
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Histochemical applications of x-ray microanalysis: the simultaneous assessment of mitosis and cell death.
pubmed:publicationType
Journal Article