Linked Life Data

7173851

Source:http://linkedlifedata.com/resource/pubmed/id/7173851

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1983-2-25
pubmed:abstractText
The utility of fluorescence microscopy in the evaluation of hematoxylin-eosin-stained sections for the presence of myocardial necrosis was examined. Sixteen human autopsy cases with clinical and pathologic evidence of myocardial infarction were studied. In addition, to better define the sensitivity and specificity of this technique, the hearts of 20 dogs that had undergone experimental coronary occlusion of known duration were studied; the duration of occlusion was three hours in seven dogs, six hours in seven dogs, and seven days in six dogs. In both human and dog studies, hypereosinophilic necrotic fibers in hematoxylin-eosin-stained sections appeared bright yellow by fluorescence microscopy. In myocardium that was shown to be necrotic by triphenyltetrazolium chloride staining and electron microscopy but was not hypereosinophilic, there was no bright yellow fluorescence of myofibers. Our study shows that bright yellow fluorescence is related to hypereosinophilia of myofibers and thus can be used to detect myocardial necrosis. However, since only hypereosinophilic fibers show the characteristic yellow fluorescence, the method appears to offer no distinct advantages over routine light microscopic techniques.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0046-8177
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1091-4
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Evaluation of fluorescence microscopy for the identification of necrotic myocardium.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.