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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1982-7-8
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pubmed:abstractText |
In studies where [1-13C]palmitic acid is employed as isotopic tracer in vivo, we have described a selected ion monitoring gas-liquid chromatography-mass spectrometry micro-method which allows plasma tracer enrichment as well as plasma palmitate content to be determined in the same 100-microliter sample through the use of [5,5,6,6-2H4]palmitic acid as assay internal standard. For standard solutions in the range equivalent to plasma palmitic acid concentrations of 10--2500 microM, assay precision was +/- 5%. For plasma samples in the physiologic range (approximately 30--200 microM palmitate) assay precision averaged better than +/- 2%. The use of the method is illustrated by measuring palmitate turnover in a 12-hr-old human infant.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Mar
|
pubmed:issn |
0022-2275
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
502-7
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7077163-Carbon Isotopes,
pubmed-meshheading:7077163-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:7077163-Humans,
pubmed-meshheading:7077163-Infant, Newborn,
pubmed-meshheading:7077163-Microchemistry,
pubmed-meshheading:7077163-Palmitic Acid,
pubmed-meshheading:7077163-Palmitic Acids
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pubmed:year |
1982
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pubmed:articleTitle |
Stable isotope dilution method for measurement of palmitate content and labeled palmitate tracer enrichment in microliter plasma samples.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|