| pubmed-article:7053368 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7053368 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:7053368 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:7053368 | lifeskim:mentions | umls-concept:C0217838 | lld:lifeskim |
| pubmed-article:7053368 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:7053368 | pubmed:dateCreated | 1982-2-25 | lld:pubmed |
| pubmed-article:7053368 | pubmed:abstractText | A sialyltransferase activity which catalyzes the synthesis of the tetrasialoganglioside GQ1b (N-acetylneuraminyl-N-acetylneuraminylgalactosyl-N-acetylgalactosaminyl [N-acetylneuraminyl-N-acetylneuraminyl]-galactosylglucosylceramide) from added trisialoganglioside GT1b (N-=acetylneuraminylgalactosyl-N-acetylgalactosaminyl [N-acetylneuraminyl-N-acetylneuraminyl]galactosylglucosylceramide) and CMP-N-acetyl[4-14C]neuraminic acid has been demonstrated using a membrane fraction of embryonic chick brain. Optimum enzymatic activity was obtained using the detergent Triton CF-54 at a pH of 6.6. Enzyme activity appeared unaffected by Ca2+, Mg2+, Mn2+, EDTA, or histone. A slight elevation in activity was seen in the presence of Hg2+. When the disialoganglioside GD1b (galactosyl-N-acetylgalactosaminyl [N-acetylneuraminyl-N-acetylneuraminyl]galactosylglucosylceramide) was used as the glycolipid substrate, approximately 15% of the radioactive label was found in GQ1b. When this GQ1b was subjected to a periodate oxidation-borohydride reduction, the distribution of radioactive label was consistent with GQ1b being the major tetrasialoganglioside product and that its synthesis could proceed via the sequence GD1b-GT1b-GQ1b. | lld:pubmed |
| pubmed-article:7053368 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7053368 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7053368 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7053368 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7053368 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7053368 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7053368 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7053368 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7053368 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7053368 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7053368 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7053368 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7053368 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:7053368 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:7053368 | pubmed:author | pubmed-author:YuR KRK | lld:pubmed |
| pubmed-article:7053368 | pubmed:author | pubmed-author:YoheH CHC | lld:pubmed |
| pubmed-article:7053368 | pubmed:author | pubmed-author:MacalaL JLJ | lld:pubmed |
| pubmed-article:7053368 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7053368 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:7053368 | pubmed:volume | 257 | lld:pubmed |
| pubmed-article:7053368 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7053368 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7053368 | pubmed:pagination | 249-52 | lld:pubmed |
| pubmed-article:7053368 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:7053368 | pubmed:meshHeading | pubmed-meshheading:7053368-... | lld:pubmed |
| pubmed-article:7053368 | pubmed:meshHeading | pubmed-meshheading:7053368-... | lld:pubmed |
| pubmed-article:7053368 | pubmed:meshHeading | pubmed-meshheading:7053368-... | lld:pubmed |
| pubmed-article:7053368 | pubmed:meshHeading | pubmed-meshheading:7053368-... | lld:pubmed |
| pubmed-article:7053368 | pubmed:meshHeading | pubmed-meshheading:7053368-... | lld:pubmed |
| pubmed-article:7053368 | pubmed:meshHeading | pubmed-meshheading:7053368-... | lld:pubmed |
| pubmed-article:7053368 | pubmed:meshHeading | pubmed-meshheading:7053368-... | lld:pubmed |
| pubmed-article:7053368 | pubmed:meshHeading | pubmed-meshheading:7053368-... | lld:pubmed |
| pubmed-article:7053368 | pubmed:meshHeading | pubmed-meshheading:7053368-... | lld:pubmed |
| pubmed-article:7053368 | pubmed:meshHeading | pubmed-meshheading:7053368-... | lld:pubmed |
| pubmed-article:7053368 | pubmed:meshHeading | pubmed-meshheading:7053368-... | lld:pubmed |
| pubmed-article:7053368 | pubmed:meshHeading | pubmed-meshheading:7053368-... | lld:pubmed |
| pubmed-article:7053368 | pubmed:meshHeading | pubmed-meshheading:7053368-... | lld:pubmed |
| pubmed-article:7053368 | pubmed:year | 1982 | lld:pubmed |
| pubmed-article:7053368 | pubmed:articleTitle | In vitro biosynthesis of the tetrasialoganglioside GQ1b. | lld:pubmed |
| pubmed-article:7053368 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7053368 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7053368 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7053368 | lld:pubmed |
