Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1982-9-10
pubmed:abstractText
The effectiveness of Micro-ID and API 20E as same-day identification systems for Enterobacteriaceae was evaluated in comparison with conventional identification by using 315 clinical isolates and 90 stock strains. The API 20E system was heavily inoculated according to manufacturer's recommendations for same-day identification. We found that 83 and 81% of isolates provided adequate inocula for Micro-ID and API 20E, respectively, and purity of the heavy inocula was not a problem with either system. Overall agreement with conventional identification at genus and species levels was 93.5% with Micro-ID and 90.2% with API 20E. However, when Klebsiella pneumoniae and K. oxytoca were considered as a single species and Proteus morganii was equated with Morganella morganii, agreement was 95.8 and 90.5%, respectively. Only 83.% of isolates were identified on the day of inoculation by API 20E, in contrast to 94.3% with Micro-ID. The remaining isolates required supplementary overnight testing. Provisional (low selectivity) determinations were constant with conventional identification with 49.3% of isolates with API 20E and 82.6% with Micro-ID. Telephone consultations with the manufacturers to resolve unprinted octal codes required a maximum of 15 min with Micro-ID and from 2 to greater than 48 h with API 20E.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
885-90
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Comparison of micro-ID and API 20E in rapid identification of Enterobacteriaceae.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't