Linked Life Data

7044761

Source:http://linkedlifedata.com/resource/pubmed/id/7044761

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1982-8-14
pubmed:abstractText
The release of newly synthesized renin by dog renal cortical slices was investigated. A radioactive protein believed to be renin was secreted from cortical slices into an incubation medium containing radioactive leucine. The nature of the radioactive protein was established by dialyzing the incubation medium after the cortical slices were removed, and subsequently applying the dialysate to 15% discontinuous polyacrylamide gels. Renin activity in the gels comigrated with the labeled protein. The labeled protein, as evidenced by a peak in 3H or 14C disintegrations per min, also comigrated with renin activity in isoelectric focusing gels. The molecular weight of the labeled protein was found to be the same as renin, approximately 40,000. Cortical or medullary slices possessing little or no renin activity did not secrete the labeled protein into the incubation medium. Isoproterenol significantly increased total renin secretion from the slices, but not the secretion of newly synthesized renin. The data support the hypothesis that two or more pools of renin are available for release. One pool releases newly synthesized renin, while another pool releases older, possibly stored renin, dependent in part on the stimuli available for renin release.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
111
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
201-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Secretion of newly synthesized renin.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't