Linked Life Data

7042845

Source:http://linkedlifedata.com/resource/pubmed/id/7042845

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1982-7-8
pubmed:abstractText
Stable human T cell hybridomas were produced by cell fusion between PHA-activated human peripheral blood lymphocytes and human acute lymphatic leukemia cells of a CEM cell line whose proliferation had been inhibited by treatment with an irreversible protein synthesis inhibitor (emetine) and an irreversible RNA synthesis inhibitor (actinomycin D). Two hybrid cell lines (E10, F8) thus produced expressed OKT3-reactive antigen, HLA-A1 and -B8 antigens derived from CEM cells, and HLA-A2 antigen derived from PBL, and they have 10 more chromosomes than CEM cells. Furthermore, these cell lines continuously secreted lymphotoxin over 3 mo. E10 was found to produce MIF in addition to lymphotoxin. Cloning of E10 and the relationship between the functions and surface phenotypes of E10 sublines were examined. Lymphotoxin-producing hybrids (E10-15 and E10-37) express more OKT8-reactive antigen than OKT4-reactive antigen, while lymphotoxin and MIF-producing hybrids (E10-25, E10-42, and E10-43) express more OKT4-reactive antigen than OKT8-reactive antigen.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:volume
128
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2714-8
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Human T cell hybridomas producing lymphokines. I. Establishment and characterization of human T cell hybridomas producing lymphotoxin and migration inhibitory factor.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't