Linked Life Data

6734633

Source:http://linkedlifedata.com/resource/pubmed/id/6734633

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-8-22
pubmed:abstractText
The phagocytic pathway for the uptake of multivalent Fc complexes by neutrophil leukocytes can be partially dissected by using a pulse-chase protocol at different chase temperatures. Utilising a deoxyribonuclease I inhibition assay, we show that an early response to recognition of phagocytic substrate by neutrophils is a rapid (less than 10 sec) polymerization of actin. Net actin assembly is transient and is reversed at higher chase temperatures which appears to allow for further progress of the pulse of phagocytic substrate along the pathway. Assembly of actin monitored in whole cells is paralleled by an increase in total actin retained in the cortical filamentous network of detergent-insoluble ghosts prepared from phagocytosing neutrophils. These data provide direct evidence for the reversible reorganisation of actin filaments in the cortical cytoplasm of phagocytic cells during phagocytosis. Furthermore, this experimental system provides an opportunity to study the characteristics of ligand-induced actin assembly, and subsequent disassembly in situ.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0171-9335
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
80-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Fc receptor-directed phagocytic stimuli induce transient actin assembly at an early stage of phagocytosis in neutrophil leukocytes.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't